| 生物反应器在金线莲扩繁中的应用 |
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| 引用本文： | 吴荣哲.生物反应器在金线莲扩繁中的应用[J].农业科学与技术,2010(9):41-44. |
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| 作者姓名： | 吴荣哲 |
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| 作者单位： | 延边大学农学院园艺系,吉林延吉133002 |
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| 摘 要： | 目的]研究在自动化低成本的生物反应器下内大量扩繁金线莲的简单方法。方法]把金线莲丛状芽培养在不同环境条件的3L球形生物反应器中,加入花宝（20-20-20）1g/L＋（6.5-4.5-19）1g/L培养基,添加蛋白胨2g/L、蔗糖3%和颗粒活性炭0.5g/L,比较b凝胶培养基与不同条件下生物反应器（浸没式、接触式和潮汐式）培养的金线莲丛生芽的生长情况。结果]植株生长（干重、茎高、叶数、根数和根长）在接触式生物反应器中结果相对较好。当外植体数目在60～90个、通气量在0.06vvm时测试结果（鲜重、茎长、叶数、叶面积和根数）最好,再生苗拥有良好的根系且在温室容易驯化成功。结论]利用生物反应器扩繁金线莲是一种新的有效途径。
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| 关 键 词： | 金线莲 球形生物反应器 扩繁 潮汐式 |
Development of a Bioreactor System for the Proliferation of Anoectochilus formosanus |
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| WU Rong-zhe.Development of a Bioreactor System for the Proliferation of Anoectochilus formosanus[J].Agricultural Science ＆ Technology,2010(9):41-44. |
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| Authors: | WU Rong-zhe |
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| Institution: | WU Rong-zhe Department of Horticulture,Agricultural College of Yanbian University,Yanji 133002 |
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| Abstract: | Objective] This study was to develop an autonomic and low cost bioreactor system for the proliferation of Anoectochilus formosanus.Method]The mulitple shoots of A.formosanus were cultured in three-liter balloon type bubble bioreactors under different conditions by adding 1.0 g/L the ready-to-use media Hyponex（20-20-20）and 1.0 g/L ready-to-use Hyponex（6.5-4.5-19）,2.0 g/L peptone,30 g/L sucrose and 0.5 g/L active carbon granules,and various gelatin media and culture methods（continuous immersion with or without bracing net and temporary immersion）were also compared.Result]In the bioreactor of continuous immersion with bracing net,A.formosanus plantlets grew best in dry weight,stem length,leaf number,root number and root length under other parameters of 60-90 explants,0.06 vvm aeration volume.The plantlets regenerated had well developed root system and were easily acclimatized in greenhouse.Conclusion]The system developed in the present study could be an effective approach to proliferate A.formosanus. |
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| Keywords: | Anoectochilus formosanus Balloon-type airlift bioreactor Proliferation Temporary immersion |
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