| 不同菌源诱导免疫的黄粉虫抗菌肽提取物对26种病原菌抑菌活性分析 |
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| 引用本文： | 谢咸升,董建臻,李静,李永利,王圆圆,李小凤,张亚南.不同菌源诱导免疫的黄粉虫抗菌肽提取物对26种病原菌抑菌活性分析[J].农业科学与技术,2010(9):1-4,9. |
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| 作者姓名： | 谢咸升 董建臻 李静 李永利 王圆圆 李小凤 张亚南 |
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| 作者单位： | [1]山西省农业科学院小麦研究所,山西临汾041000 [2]河北农业大学植物保护学院,河北保定071001 |
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| 基金项目： | 河北省自然科学基金项目（C200700450）. |
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| 摘 要： | 目的]探索经不同菌源诱导黄粉虫所产生的免疫物质——抗菌肽对病原菌是否存在活性差异。方法]对饥饿后黄粉虫幼虫通过饲细菌、针刺饲真菌（放线菌）法产生诱导免疫反应,研磨、冷冻离心法获得抗菌肽提取物,采用抑菌圈法测定其对26种病原菌抑菌活性,利用三等分法划分四种类型评价抑菌活性及抑菌谱。结果]经不同菌源诱导黄粉虫所产生的抗菌肽抑菌活性及抑菌谱上均存在明显差异。结论]生防菌源在诱导昆虫体液免疫物质——抗菌肽表达上具有明显优势。
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| 关 键 词： | 黄粉虫 诱导免疫反应 抗菌肽 抑菌活性 抑菌谱 |
Analysis of the Antimicrobial Activity of Antimicrobial Peptides Extracted from Immunized Tenebrio molitor Induced by Different Microbial Treatments Against 26 Pathogens |
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| XIE Xian-sheng,DONG Jian-zhen,LI Jing,LI Yong-li,WANG Yuan-yuan,LI Xiao-feng,ZHANG Ya-nan.Analysis of the Antimicrobial Activity of Antimicrobial Peptides Extracted from Immunized Tenebrio molitor Induced by Different Microbial Treatments Against 26 Pathogens[J].Agricultural Science ＆ Technology,2010(9):1-4,9. |
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| Authors: | XIE Xian-sheng DONG Jian-zhen LI Jing LI Yong-li WANG Yuan-yuan LI Xiao-feng ZHANG Ya-nan |
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| Institution: | 1.Wheat Research Institute,Shanxi Academy of Agricultural Sciences,Linfen 041000;2.College of Plant Protection,Agricultural University of Hebei,Baoding 071001 |
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| Abstract: | Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor varied with different pathogens as inducers.Method]By inducing T.molitor hungry larvaes to generate immune response via feeding with bacteria and with fungi or actinomycete post to pricking,the antimicrobial peptides extracts were obtained by grinding and centrifuging the cultures.Its antimicrobial activity against 26 pathogens was measured by bacteriostatic ring,and evaluated by trisection to four types and inhibitory spectrum.Result]Both the antimicrobial activity and antimicrobial spectrum of the antimicrobial peptides extracts varied remarkably among different pathogens as inducers.Conclusion]Bio-control used strains have obvious advantage in inducing the insect to express body fluid immunity material-antimicrobial peptides. |
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| Keywords: | Tenebrio molitor Induced immune response Antimicrobial peptides Antimicrobial activity Antimicrobial spectrum |
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