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| 动物饲料中植物源性外源转基因成分的检测 | |
| 引用本文： | 金红,赵昕,吴疆,韦东胜,刘梦颖,王永.动物饲料中植物源性外源转基因成分的检测[J].华北农学报,2006,21(5):54-59. |
| 作者姓名： | 金红 赵昕 吴疆 韦东胜 刘梦颖 王永 |
| 作者单位： | 1. 天津农学院,农学系,天津,300384 2. 辽宁出入境检验检疫局,辽宁,大连,116001 3. 天津市农业科学院中心实验室,天津,300192 |
| 基金项目： | 天津市农业科学院院长基金 |
| 摘 要： | 针对宠物饲料、奶牛饲料和浓缩饲料等不同类型饲料的特点,采取相应的不同DNA提取方法,通过对组成饲料的所有作物成分相应内源基因的检测,确定待测饲料样品的作物组成成分,并建立了各类饲料中转基因成分的检测技术。有针对性地论述了饲料检测中可能出现的问题、技术难点和关键技术,为实际检验工作提供可借鉴的建议。 |
| 关 键 词： | 植物源性 饲料 DNA提取方法 PCR检测 内源基因 转基因成分 |
| 文章编号： | 1000-7091（2006）05-0054-06 |
| 收稿时间： | 2005-07-14 |
| 修稿时间： | 2005-07-14 |
Studies on the Techniques for Detection of Transgenic Foreign Genes Originated from Plants in Animal Feed |
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| JIN Hong,ZHAO Xin,WU Jiang,WEI Dong-sheng,LIU Meng-ying,WANG Yong.Studies on the Techniques for Detection of Transgenic Foreign Genes Originated from Plants in Animal Feed[J].Acta Agriculturae Boreali-Sinica,2006,21(5):54-59. | |
| Authors: | JIN Hong ZHAO Xin WU Jiang WEI Dong-sheng LIU Meng-ying WANG Yong |
| Institution: | 1 .Tianjin Agricultural College, Tianjin 300384, China; 2. Liaoning Bureau of Import and Export Detection and Inspection, Dalian 116001, China; 3. Central Lab of Tianjin Academy of Agricultural Sciences, Tianjin 300192, China |
| Abstract: | Because of several reasons such as big difference of components among different kinds of animal feed,degradation of DNAs from raw materials and addition of a large amount of supplemental components for e.g.vitamins and trace elements,it becomes difficult to isolate DNAs from animal feed.How to isolate DNA efficiently aimed to different kinds of animal feed using different methods is the hard point.Our target is to establish DNA isolation methods.Due to the complicated situation of animal feed components,for e.g.animal feed from different agricultural producing areas,the component may be highly different,so,firstly we need to figure out clearly kinds of component by PCR-detecting the corresponding native genes of all possible transgenic crops contained in animal feed before detecting the foreign transgenic factors.The detection techniques were established using pet feed,milk cow feed and three kinds of concentrated feed as samples,and using PCR as the main detection method.This thesis specifically discussed the possibly appeared problems,technique difficulties and key techniques in the detection of animal feed,which will be very helpful to the actual detection work. |
| Keywords: | Plants originated Feed DNA isolation method PCR detection Native gene Transgenic foreign gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |