| 不同月份厚朴叶多糖体外清除羟自由基作用 |
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| 引用本文： | 邓芬,张芳芹,张家蓉,马毓,汤贵滋,刘存芳,赖普辉.不同月份厚朴叶多糖体外清除羟自由基作用[J].安徽农业科学,2011,39(36):22303-22304. |
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| 作者姓名： | 邓芬 张芳芹 张家蓉 马毓 汤贵滋 刘存芳 赖普辉 |
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| 作者单位： | 1. 陕西理工学院化学与环境科学学院,陕西汉中,723000
2. 陕西理工学院生物科学与工程学院,陕西汉中,723000 |
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| 基金项目： | 陕西理工学院2011年大学生创新实验项目(UIRP1131) |
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| 摘 要： | 目的]研究不同月份厚朴叶多糖体外对羟自由基（·OH）的清除作用。方法]以不同月份厚朴叶为原料，通过提取及分离纯化得到厚朴叶多糖，采用分光光度法测定不同月份厚朴叶多糖对Fenton反应产生的羟自由基（·OH）的清除能力，并与抗坏血酸（Vc）的抗氧化性进行了比较。结果]不同月份厚朴叶多糖对羟自由基的清除率随多糖浓度的增加而增强；与抗坏血酸（Vc）比较，厚朴叶多糖具有更强的清除羟自由基能力，8月份厚朴叶多糖的半抑制率圮，。为0．08g／L，相当于O．12g／Lvc对羟自由基的清除率；同等浓度条件下，10月份厚朴叶多糖在较高浓度时比抗坏血酸（Vc）的抗氧化活性更高。结论]不同月份厚朴叶多糖在受适浓度范围内对羟自由基有明显的抑制作用
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| 关 键 词： | 厚朴叶 多糖 羟自由基 分光光度法 |
Study on Effect of Polysaccharide from Magnolia Leaves in Different Months on Scavenging Hydroxyl radical in Vivo |
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| Institution: | DENG fen et al (School of Chemistry and Environmental Sciences,Shaaxi University of Technology,Hanzhong,Shaanxi 723000) |
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| Abstract: | Objective] The research aimed to analyze the effect of the polysaccharide from the magnolia leaves in different months on scavenging the hydroxyl radical(·OH) in vivo.Methods] With the magnolia leaves in different months as the tested materials,the polysaccharide from the magnolia leaves was obtained through the extraction,separation and purification.The ability of the polysaccharide from different months to remove the hydroxyl radical(·OH) produced by Fenion reaction was determined by the spectrometry and was compared with the oxidation resistance of V_C.Results] The scavenging rate of the polysaccharide from the magnolia leaves in different months on the hydroxyl radical was enhanced with the increment of the polysaccharide concn.Compared with the V_C,the polysaccharide from the magnolia leaves had the greater ability to remove the hydroxyl radical.The half inhibition rate IC_50 of the polysaccharide from the magnolia leaves in August was 0.08 g/L,being equivalent to the scavenging rate of 0.12 g/L V_C agaisnt the hydroxyl radicals.Under the condition of the same concn.,the polysaccharide at higher concn.from the magnolia leaves in October was higher than the V_C on the antioxidation activity.Conclusion] The polysaccharide from the magnolia leaves in different months had obvious inhibition on the hydroxyl radicals in the tested concn.range. |
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| Keywords: | Magnolia leaves Polysaccharide Hydroxyl radical Spectrophotometry |
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