| TDZ在观音莲组织培养中的应用 |
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| 引用本文： | 陈荣,李庆玲,朱昌叁.TDZ在观音莲组织培养中的应用[J].安徽农业科学,2011,39(36):22224-22225. |
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| 作者姓名： | 陈荣 李庆玲 朱昌叁 |
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| 作者单位： | 广西生态工程职业技术学院,广西柳州545004;华南农业大学生命科学院药用植物研究中心,广东广州510642;华南农业大学生命科学院药用植物研究中心,广东广州,510642;广西生态工程职业技术学院,广西柳州,545004 |
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| 基金项目： | 广西自然科学基金资助(2010GXNSFB013024) |
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| 摘 要： | 目的]研究TDZ及IBA2种植物生长调节物质在观音莲组培生产中的应用。方法]以观音莲（Alocasia indica Schott）茎为外植体，通过改变培养基激素浓度及不同激素配比，比较它们对观音莲芽增殖和生根的影响。结果]以MS＋3％蔗糖＋0．6％琼脂＋0．1mg／L肌醇为芽增殖基本培养基，pH5．6～5．8，0．20mg／LTDZ＋0．2mg／LIBA时取得了最大的增殖系数，在实际生产中两种激素浓度可以在O．2～0．4mg／L间变动。观音莲生根容易，在增殖培养时可见部分芽有根着生，以1／2MS＋1．5％蔗糖＋0．6％琼脂＋0．1mg／L肌醇为生根基本培养基，添加0．2mg／LIBA可以缩短生根时间，根系质量高，移栽容易成活。结论]建立了更高繁殖系数的观音莲组培方法，为这一重要花卉的工厂化育苗提供了技术参考。
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| 关 键 词： | 观音莲 TDZ 组织培养 |
Study on Application of TDZ in Tissue Culture of Alocasia indica |
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| Institution: | CHEN Rong et al (Guangxi Eco-engineering Vocational the Technical College,Liuzhou,Guangxi 545004) |
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| Abstract: | Objective] The aim was to analyze the application of TDZ and IBA in tissue culture of Alocasia indica.Method] Using Alocasia indica Schott stems as explants,changing the hormone levels and different hormone combinations in medium to compare them influence to the Alocasia indica bud proliferation and rooting.Result] Most suitable for bud propagation nutrient medium ingredient was MS+3% Sugar+0.6% Agar+ 0.1 mg/L myoinositol,pH 5.6-5.8.Combination of TDZ 0.20 mg/L with IBA 0.1 mg/L was most efficient for bud propagation.In actual production,concentration of TDZ and IBA could vary between 0.2 mg/L and 0.4 mg/L.Rooting of Alocasia indica was easy.1/2MS+1.5% Sugar+0.6% Agar+0.1 mg/L myoinositol were most suitable for rooting.Adding 0.2-0.4 mg/L IBA could shorten rooting time and improved quality of root system.Sedlings were also easily surving in transplanting.Conclusion] A higher propagation coefficient of Alocasia indica tissue culture method was established,providing technical reference for factory nursery of this important flowering plant. |
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| Keywords: | Alocasia indica TDZ Culture |
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