| 水稻愈伤组织液体增殖培养的初步研究 |
| |
| 引用本文： | 林艺.水稻愈伤组织液体增殖培养的初步研究[J].安徽农学通报,2010,16(11):60-62,88. |
| |
| 作者姓名： | 林艺 |
| |
| 作者单位： | 宿州市农业科学研究所科技管理科,安徽宿州,234000 |
| |
| 摘 要： | 以MS培养基为基本培养基,采用液体培养的方式,通过调节2,4-D,6-BA及蔗糖浓度,对粳稻南农4007愈伤组织的增殖进行了初步研究。结果表明：2,4-D浓度在0.0～4.0mg/L时对水稻愈伤组织的增殖和生长有一定的影响,6-BA浓度在0.2～3.2mg/L、蔗糖浓度在10～50g/L时对愈伤组织增殖生长差异不显著,但当2,4-D为2.0mg/L、6-BA为0.8mg/L,蔗糖为20g/L时,其愈伤组织不论从生长状态,还是增殖速度上都较好且褐化程度相对较轻。适合粳稻南农4007愈伤组织液体增殖培养的基本配方为MS＋NAA0.5mg/L＋2,4-D2.0mg/L＋水解络蛋白1g/L＋蔗糖20g/L＋6-BA0.8mg/L。
|
| 关 键 词： | 水稻 愈伤组织 增殖 液体培养 |
Preliminarily Study On Rice Callus Propagation in Liquid Culture |
| |
| Lin Yi.Preliminarily Study On Rice Callus Propagation in Liquid Culture[J].Auhui Agricultural Science Bulletin,2010,16(11):60-62,88. |
| |
| Authors: | Lin Yi |
| |
| Institution: | Lin Yi ( Department of Science , technology management,Suzhou Agricultural Science Research Institute,Suzhou 23400,China) |
| |
| Abstract: | Calli of rice Nannong 4007 were cultured in the MS liquid media with different concentrations of hormones ( 2,4 - D,6 - BA) and sucrose to study their proliferation. The results showed that 2,4 - D has obvious influences on the growth of rice calli within the concentration 0. 0 - 4. 0mg/L in the liquid media. However ,6 - BA has not obvious effects on calli pro- liferation within the concentration 0. 2 - 3. 2mg/L . From rice callus growth point of view ,the sucrose had not obvious influence on rice calli wi... |
| |
| Keywords: | Rice Callus Liquid culture Tissue culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
