| 金银花提取物体外抗猪繁殖与呼吸综合征病毒研究 |
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| 引用本文： | 陈峰,江玉婷,于云飞,王桂萍,邹坤,陈剑锋.金银花提取物体外抗猪繁殖与呼吸综合征病毒研究[J].动物医学进展,2012(2):41-45. |
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| 作者姓名： | 陈峰 江玉婷 于云飞 王桂萍 邹坤 陈剑锋 |
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| 作者单位： | 天然产物研究与利用湖北省重点实验室(三峡大学);三峡大学化学与生命科学学院 |
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| 基金项目： | 三峡大学博士启动基金(KJ2008B014) |
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| 摘 要： | 通过观察金银花提取物对猪繁殖与呼吸综合征病毒(PRRSV)感染的Marc-145细胞的保护效果、对病毒感染滴度(TCID50)的影响及对ORF7mRNA表达的影响来评价其体外抗猪繁殖与呼吸综合征病毒的活性。结果表明,金银花提取物大孔树脂600mL/L的乙醇/水洗脱部位对PRRSV感染细胞具有较好的保护效果,最小保护浓度为6.25μg/mL。6.25μg/mL 600mL/L的乙醇/水洗脱部位使PRRSV病毒滴度从105.8 TCID50降低至100.3 TCID50左右,使PRRSV ORF7mRNA含量降低1 000多倍。因此,金银花提取物大孔树脂600mL/L的乙醇/水洗脱部位具有良好的体外抗PRRSV活性。
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| 关 键 词： | 金银花 猪繁殖与呼吸综合征病毒 Marc-145 |
Study on Anti-PRRSV Effect of Lonicera japonica Extracts in Vitro |
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| CHENG Feng,JIANG Yu-ting,YU Yun-fei,WANG Gui-ping,ZOU Kun,CHEN Jian-feng.Study on Anti-PRRSV Effect of Lonicera japonica Extracts in Vitro[J].Progress In Veterinary Medicine,2012(2):41-45. |
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| Authors: | CHENG Feng JIANG Yu-ting YU Yun-fei WANG Gui-ping ZOU Kun CHEN Jian-feng |
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| Institution: | (Hubei Key Laboratory of Natural Products Research and Development,College of Chemistry and Life Science,China Three Gorges University,Yichang,Hubei,443002,China) |
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| Abstract: | The antiviral effect of Lonicera japonica on PRRSV in vitro was evaluated by observing cytopathic effect(CPE) on PRRSV infected Marc-145 cell,determining TCID50 and evaluating ORF7 mRNA expression level.The results showed that the 600 mL/L ethanol/water elution fractions possess the perfect protective effect on PRRSV infected cells and with the minimum protective concentration of 6.25 μg/mL.The 6.25 μg/mL 600 mL/L ethanol/water elution fractions decreased PRRSV titer from 105.8 TCID50 to 100.3 TCID50 and reduced the PRRSV ORF7 mRNA contents about 1 000 times.In summary,we disclosed 600 mL/L ethanol/water elution fractions of Lonicera japonica as a potential antiviral drug owing to its perfect anti-PRRSV effect. |
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| Keywords: | Lonicera japonica PRRSV Marc-145 cell |
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