Phenotypic and genotypic properties of Escherichia coli isolated from colisepticemic cases of Japanese quail |
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| Authors: | Salehi Mahmood Ghanbarpour Reza |
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| Institution: | (1) Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, P.O. Box 7616914111, Kerman, Iran;(2) Department of Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran |
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| Abstract: | This study was conducted to characterize the Escherichia coli isolates from colisepticemic Japanese quails. One hundred and nine E. coli were isolated in pure culture from heart blood of dead Japanese quails. The sampled birds were originated from four different
farms. Antibiotic resistance pattern of E. coli isolates were determined against nine antibacterial agents. Phylotype and virulence genes of the isolates were detected by
polymerase chain reaction. By disc diffusion method, all of the isolates showed resistance to three or more antibiotics, and
19 different patterns of multiple drug resistance were observed. Phylotyping of the most prevalent multiple drug-resistant
isolates revealed that they mostly belonged to phylogroups A (A1 subgroup). The E. coli isolates belong to four phylogenetic groups: A (55.0%), B1 (18.3%), B2 (17.4%), and D (9.2%). Eighty-nine (81.7%) isolates
were distributed in five phylogenetic subgroups including 22 (20.2%) in A0, 38 (34.9%) in A1, 19 (17.4%) in B23, 7 (6.4%) in D1, and 3 (2.8%) in D2. The examined E. coli isolates exhibit at least one of the virulence genes tested, whereas three most prevalent genes were crl (94.5%), fimH (89.0%), and iutA (51.4%), respectively. The genetic marker for Afa (afaI B-C), S (sfa/focD-E), and P (papE-F) fimbriae were found in one, four, and ten isolates, respectively. Thirteen different combinations of virulence gene were
observed, where combination of crl and fimH genes was the most prevalent pattern. None of the isolates contained the ipaH, stx1, stx2, and eaeA genetic markers. In conclusion, E. coli strains could be considered as a causative agent of mortality in quail farms. In conclusion, E. coli isolates from colisepticemic quails are distributed in different phylogroups, are resistant to combinations of antibiotic
agents, and contain several virulence genes. |
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