| 猴嗜T淋巴细胞病毒1型核酸快速检测方法的建立 |
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| 引用本文： | 熊炜,蒋静,张强,盘宝进,李健,魏晓锋,黄忠荣,胡建华.猴嗜T淋巴细胞病毒1型核酸快速检测方法的建立[J].动物检疫,2013(9):64-67. |
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| 作者姓名： | 熊炜 蒋静 张强 盘宝进 李健 魏晓锋 黄忠荣 胡建华 |
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| 作者单位： | [1]上海出入境检验检疫局,上海200135 [2]广西出入境检验检疫局,广西南宁530028 [3]上海实验动物研究中心,上海201203 |
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| 基金项目： | 上海市科委科研项目（编号11140901100） |
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| 摘 要： | 猴嗜T淋巴细胞病毒l型（Simian T-cell lymphotropic virus type 1,STLV-1）是无特定病原体（SPF）猴必须排除的病毒之一,其潜伏期较长,主要侵害猴的免疫系统。为强化口岸对进出境野生及实验用灵长类动物STLV-1感染情况的监测和流行病学调查,本研究建立了RT-PCR和Real-time RT-PCR检测STLV-1的方法,并对方法的特异性、敏感性和稳定性进行了确认。
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| 关 键 词： | 猴 猴嗜T淋巴细胞病毒1型 RT-PCR Real-time RT-PCR Taqman探针 |
Establishment of rapid nucleic acid detection methods for Simian T-cell lymphotropic virus type 1 |
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| Authors: | Xiong Wei Jiang Jing Zhang Qiang Pan Baojin Li Jian Wei Xiaofeng Huang Zhongrong Hu Jianhua |
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| Institution: | 1. Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135： 2. Guangxi Entry-Exit Inspection and Quarantine Bureau, Nanning 530028; 3. Shanghai Laboratory Animal Research Center, Shanghai 201203) |
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| Abstract: | Simian T-cell lymphotropic virus type 1（STLV-1） is one of viruses required to be free in specefic pathogen free（SPF） monkey.The virus has a long infective period and damages the immune system of monkey. In order to survey and investigate the epidemic characters of STLV-1 transmission among wild and experimental monkeys,methods of RT-PCR and Real-time RT-PCR using TaqMan probe to detect STLV-1 were established and proved to be specific with high sensitivity and stability. |
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| Keywords: | monkey simian T-cell lymphotropic virus type 1 RT-PCR real-time RT-PCR Taqman probe |
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