Analysis of the Cecum Microbial Diversity of Different Pig Breeds by Illumina Amplicon Sequencing of 16S rDNA Tag |
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| Authors: | KANG Run-min LI Yao LV Xue-bin JI Gao-sheng YING San-cheng ZENG Kai LI Yan YIN Ming-yu |
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| Institution: | 1. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China;
2. Key Laboratory of Bio-resources and Ecoenvironment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, China |
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| Abstract: | To study the diversity and composition of the bacterial community from cecum samples from Yacha pig, Qingyu pig and Wujin pig, the investigation was designed to reveal mechanism of roughage resistance. Illumina amplicon sequencing of 16S rDNA Tag was used to analyze the cecum microbial diversity of Yacha pig, Qingyu pig and Wujin pig at 90 kg liveweight. The results indicated that the core flora of the three breeds were Bacteroidetes and Firmicutes at the phylum level and Prevotella and Bacteroides at the genus level. Meanwhile bacteria associated with cellulose decomposition were found in all three breeds, in which the number of Fibrobacter and Clostridium in Yacha pig were significantly higher than that in Qingyu pig and Wujin pig (P<0.05),and the number of Prevotella in Yacha pig was significantly higher than that in Wujin pig (P<0.05). The number of Bacteroides in Qingyu pig was significantly higher than that in Yacha pig (P<0.05).The number of Spirochaetes in Qingyu pig was significantly higher than that in Wujin pig (P<0.05). It could conclud that the composition of the cecum bacteria community was similar in above pig breeds, but there were significant differences in distribution and quantity. According to analysis of microbial related to cellulose digestibility, Yacha pig was the strongest among the three breeds, followed by the Qingyu pig and Wujin pig. |
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| Keywords: | Yacha pig Qingyu pig Wujin pig cecum microbial diversity cellulose digest ability |
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