Study on Cultivation Research and Preliminary Application of Modern Commercial Piglet Kidney Cell Strain |
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| Authors: | HU Hong-hui WU Jing-bo HUANG Jian-qiang NAN Wen-jin YU Wen-tao |
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| Institution: | Animal Disease Laboratory, Shaoguan University, Shaoguan 512005, China |
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| Abstract: | Cell line was an important carrier for the isolation and culture of animal viruses.In the research, modern commercial piglet kidney was taken as the raw material in order to cultivate a new cell line for isolating and culturing animal viruses. By trypsin digestion and differential velocity adherent combination method,porcine kidney epithelial cells were separated and purified,and then continuously subcultured in vitro. The results showed that a new cell strain named SDPK-D had been serially passaged for 90 generations.The doubling time of SDPK-D cell strain F33 and F83 were 40.9 and 32.7 h,respectively;While the cell viability were 97.55% and 98.86%,the cell adherent rate at the 8 h were 91.67% and 97.06%,respectively. Obvious cytopathic effect appeared after inoculating with PRV,SIV and PEDV positive samples. For the first time, a new cell strain from modern pig named SDPK-D was successfully cultivated,which was sensitive for several animal viruses.It could provid a new selection for the isolation and culture of virus. |
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| Keywords: | piglet kidney cell primary culture subculture cell strain |
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