Study on Colloidal Gold Immunochromatographic Assay for Rapid Detection of Estradiol in Milk |
| |
| Authors: | BAI Yu ZHANG Jing HU Jing-yan XU Yong-peng WANG Shu-ming |
| |
| Institution: | 1. College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China;
2. Laboratory of Agricultural Products Quality Safety Risk Assessment, Ministry of Agriculture, Analysis and Test Center, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China;
3. Hangzhou Testsea Biotechnology Co., Ltd., Hangzhou 311100, China |
| |
| Abstract: | In order to develop a sensitive and specific method for the detection of estradiol residues (E2) in milk, a colloidal gold immunochromatographic method was established. Colloidal gold particles were prepared by trisodium citrate reduction method and labeled with rabbit polyclonal antibodies (PcAb) by physical adsorption method. After optimization of reaction conditions such as the amount of coating antigen and labeled antibody to assemble test strip, the sensitivity, specificity, repeatability and accelerated preservation of the test strip were determined. The estrogen analogue cross reaction and milk matrix interference reaction were also measured. The results showed that the optimized concentration of E2-OVA antigen was 2 mg/mL and the optimized antibody concentration was 20 μg/mL, visual detection limits of E2 was 10 μg/L in PBS within 10 min. The cross reaction rate of this method with estriol was 40%, there were no negligible cross-reactivities with other estrogen compounds including estradiol valerate, estradiol benzoate, estrone, diethylstilbestrol, quinestrol, ethinyloestradiol and nonylphenol. Milk samples only needed two times diluted before analysis, and the results could judge by naked eye after 10 min with cut-off of 20 μg/L. The results demonstrated that the developed method was suitable for rapid on-site screening of E2 residues in milk samples. |
| |
| Keywords: | estradiol (E2) colloidal gold immunochromatography assay rapid detection milk |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
