| 山东地区茄子感染番茄褪绿病毒的分子鉴定 |
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| 引用本文： | 刘永光,代惠洁,杨园园,陈鹏,竺晓平,赵静.山东地区茄子感染番茄褪绿病毒的分子鉴定[J].中国蔬菜,2017,1(9):41-46. |
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| 作者姓名： | 刘永光 代惠洁 杨园园 陈鹏 竺晓平 赵静 |
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| 作者单位： | 1.潍坊科技学院，山东省高校设施园艺重点实验室，山东寿光 262700；;2.山东农业大学植物保护学院，山;
东省蔬菜病虫生物学重点实验室，山东泰安 271018 |
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| 基金项目： | 山东省自然科学基金项目（ZR2015CQ022），潍坊市科技发展计划项目（2016ZJ1113），山东省农业重大应用技术创新课题 |
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| 摘 要： | 为明确山东地区番茄褪绿病毒(Tomato chlorosis virus,To CV)侵染茄子的情况,采用RT-PCR分子检测鉴定疑似感染To CV的茄子叶片样品及发病茄子植株上的烟粉虱(Bemisia tabaci)体内To CV的带毒情况。结果表明:10份茄子叶片样品中有6份扩增得到约463 bp的特异条带,经测序与Gen Bank中To CV序列相似性达到99.0%以上,To CV检出率为60%;系统进化分析显示,山东寿光地区To CV茄子分离物cp-1与北京番茄分离物BJ亲缘关系最近。温室内发病茄子植株上的烟粉虱体内携带To CV,带毒率为70%。
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| 关 键 词： | 山东地区 茄子 番茄褪绿病毒 分子检测 烟粉虱 传毒 |
Molecular Identification of Tomato chlorosis virus on Eggplant in Shandong Province |
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| LIU Yong-guang,DAI Hui-jie,YANG Yuan-yuan,CHEN Peng,ZHU Xiao-ping,ZHAO Jing.Molecular Identification of Tomato chlorosis virus on Eggplant in Shandong Province[J].China Vegetables,2017,1(9):41-46. |
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| Authors: | LIU Yong-guang DAI Hui-jie YANG Yuan-yuan CHEN Peng ZHU Xiao-ping ZHAO Jing |
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| Institution: | 1.Weifang College of Science & Technology，Key Laboratory of Protected Horticulture for Universities in Shandong Province，Shouguang 262700，Shandong，China；;2.College of Plant Protection，Shandong Agricultural University，Key Laboratory for Biology of Vegetable Diseases and Insect Pests in Shandong Province，Tai’an 271018，Shandong，China |
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| Abstract: | In order to clarify the occurrence of Tomato chlorosis virus（ToCV）on eggplant in Shandong
Province，this paper detected and identified by RT-PCR the samples of eggplant leaves prospectively infected
by ToCV and also the viruliferous percentage of ToCV in Bemisia tabaci adults on infected eggplant plants．The
results showed that 463 bp specific fragments were amplified from the detected samples and 6 samples were positive
for ToCV and the positive percentage was 60%．The CP gene of positive samples were cloned and sequenced，
sharing over 99.0% nucleotide identity with a tomato isolate from Beijing（BJ）．Moreover，ToCV could be
detected in B. tabaci on ToCV-infected eggplant plants and the viruliferous percentage of ToCV was 70%． |
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| Keywords: | Regions in Shandong Province Eggplant Tomato chlorosis virus（ToCV） Molecular identification Bemisia tabaci Viral transmission |
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