Isolation,Identification and Phylogenetic Analysis of Important Functional Genes of Porcine Pseudorabies Virus LC Strain |
| |
| Authors: | LIU Zhi-cheng LI Xin-jian ZHANG Jian-feng SHEN Hai-yan SUN Jun-ying ZHANG Chun-hong |
| |
| Institution: | 1. Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Open Laboratory of Veterinary Public Health, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Guangzhou South China Biological Medicine Co., Ltd., Guangzhou 511300, China |
| |
| Abstract: | To find out the reason of the reproductive failure in pregnant sows in a hoggery in Guangdong, the mixture with brain, lymph nodes and lungs of farm abortion stillbirth were identified by PCR, virus isolation and culture, tissue culture infective dose (TCID50) assay, homology and phylogenetic analysis of the important functional genes (gB, gC, gD and gE) and animal test. The results showed that the mixture was proved to be porcine pseudorabies virus (PRV) positive samples. The typical cytopathogenic effect was induced in the third passage of Vero cell and the titer of the fifth passage was 10-6.8/0.1 mL. The sequence analysis and phylogenetic relationship of gB, gC, gD and gE genes showed that it was a Chinese PRV variant, which was named as LC strain. The typical pseudorabies clinical and pathological symptoms were presented in 12-week-old piglets inoculated with LC strain. The results demonstrated that a local pseudorabies virus had been isolated, suggesting that the Bartha-K61 vaccine was not fully effective for controlling the current epidemic of pseudorabies in China. |
| |
| Keywords: | porcine pseudorabies virus (PRV) isolation and identification phylogenetic analysis |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
