Sequence Analysis of S Gene of 6 Strains of Porcine Epidemic Diarrhea Virus from Guizhou |
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| Authors: | MAO Li-hong ZHANG Shuang-xiang ZHOU Bi-jun WANG Wei HU Xing-yi CHENG Zheng-tao WEN Ming WANG Kai-gong |
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| Institution: | 1. Huaxi District Agricultural Bureau of Guizhou, Guiyang 550001, China;
2. Animal Disease Control and Prevention Center of Guizhou, Guiyang 550001, China;
3. College of Animal Science, Guizhou University, Guiyang 550025, China |
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| Abstract: | In order to understand the characteristics of S gene of porcine epidemic diarrhea virus (PEDV) in Guizhou province, and grasp the genetic variation of PEDV, according to 3 pairs of specific primers designed in the test, S gene of 6 strains of PEDV from Guizhou were amplified by RT-PCR, cloned and sequenced. Nucleotide sequence and phylogenetic tree of 6 strains of PEDV from Guizhou and reference strains were analyzed by biological information software. The result showed that the length of S gene genome of 6 strains of PEDV from Guizhou was 4 161 bp, encoding 1 387 amino acids, and the nucleotide homologies of domestic and foreign PEDV reference were from 93.3% to 98.8%, the amino acid identity were from 91.6% to 98.9%. Phylogenetic tree analysis results showed that the 6 strains were close to the American strain, Vietnam strain and the Shandong strain.They were far from the CV777, LZC, Brl and 83P-5.The experiment showed that the S gene of PEDV in Guizhou had a certain degree of amino acid change in recent year with a trend of variation. The study provided a theoretical basis for the prevention and control of PEDV in Guizhou province. |
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| Keywords: | porcine epidemic diarrhea S gene bioinformatics analysis |
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