Residue Analysis of Five Kinds of Penicillins in Eggs by High Performance Liquid Chromatography-Ultraviolet Detection |
| |
| Authors: | LI Lei-ting LIU Jun-hua LIU Bao-tao FU Hong-lei ZOU Ming |
| |
| Institution: | 1. College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China;
2. Qingdao Inspection Station of Feed and Veterinary Drug, Qingdao 266100, China |
| |
| Abstract: | This study was aimed to develop a method for simultaneous determination of five kinds of penicillins in eggs by high performance liquid chromatography-ultraviolet detection (HPLC-UVD). The eggs samples were extracted by acetonitrile,degreased by n-hexane,concentrated by rotary evaporation,derivatization,and then was separated by HPLC. The results showed that the calibration curves of five kinds of penicillins were linear correlated in the concentration ranges of 10 to 1 000 μg/kg with the correlation coefficients >0.999,which the limits of detection and quantification were 1.5 μg/kg (S/N=3) and 5.0 μg/kg (S/N=10),respectively. The average recoveries were 62.04% to 93.13%,the relative standard deviations were 3.91% to 13.69%.And the intra-day and inter-day RSDs were 3.98% to 14.71% and 4.26% to 12.71%,respectively. The developed method was suitable for detecting five kinds of penicillins residues in eggs with high repeatability and sensitivity. |
| |
| Keywords: | penicillin high performance liquid chromatography-ultraviolet detection (HPLC-UVD) drug residues eggs |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
