Effect on Proliferation of Goat Pox Virus of TK Gene Deficiency |
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| Authors: | LI Ji-dong CAI Xue-peng |
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| Institution: | 1. School of Agriculture, Ningxia University, Yinchuan 750021, China;
2. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
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| Abstract: | In this study, the proliferation of goat pox virus (GPV) was researched using the recombinant GPV of TK gene insertion inactivation. Different length DNA fragments X1, X2, X3 and X4 were inserted into the transfer plasmid pTKfpgigp to constructed the recombinant transfer plasmids and these plasmids were transfected into lamb testis cells infected GPV. Recombinant GPVs of TK gene inactivation by inserting foreign DNA fragment of 2 800, 4 000, 5 200 and 7 700 bp,respectively, were generated via homologous recombination. Four recombinant GPVs (rGPV/tk-) were obtained by selective culture and the virus titer were mensurated by 50% tissue culture infective dose. The results showed that the rGPV/tk- virus titers were decreased obviosuly which was comparable with the wild type GPV AV41. Furthermore, the virus titer of the recombinant virus was decreased more obviously with the extension of the foreign DNA fragment. The result indicated that the proliferation of the GPV was depressed when GPV TK gene was deficient. |
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| Keywords: | goat pox virus TK gene homologous recombination virus titer |
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