Bioinformatics Analysis of gB Gene of Porcine Lymphotropic Herpesviruses Strain GD27 |
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| Authors: | ZHU Shi-kang TONG Tie-zhu LIU Xing LI Chun-ping CHEN Yan-zhong LUO Zhuo-jun LIN Zhi-xiong LIU Zhong-yong ZHOU Yu |
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| Institution: | 1. Huizhou Entry-exit Inspection and Quarantine Bureau of P. R. C, Huizhou 516006, China;
2. Guangdong Entry-exit Inspection and Quarantine Bureau of P. R. C, Guangzhou 510623, China |
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| Abstract: | The glycoproteins B (gB) gene of porcine lymphotropic herpesviruses 2 (PLHV-2)was amplified by PCR and sequenced, then bioinformatics analysis were conducted. The gB protein was composed of 876 amino acids, the molecular formula was C4500H6975N1199O1351S40, the molecular mass was 100.77 ku, the value of theoretical isoelectric point was 6.45, and the instability index was 38.58. The prediction of secondary structure revealed that some alpha helixes and beta sheets exist in protein while random coil was major pattern. It had a signal peptide in the position 1-39 amino acids and a transmembrane helice in the position 761-780 amino acids. It contained several prosites and several intrinsically disordered proteins. Tertiary structure model of gB protein showed a fusion loop. Multiple antigenic epitope located in gB through comprehensive analysis prediction method. It was suggested that gB gene could be as a candidate antigen for vaccination of PLHV-2. |
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| Keywords: | porcine lymphotropic herpesviruses gB gene bioinformatics analysis |
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