Effects of Smo gene silencing on cell activity and apoptosis of human cervical carcinoma HeLa cells |
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| Authors: | SONG Ling ZHOU Qiang LI Na YU Jie LI Yang ZHANG Chi |
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| Institution: | 1. Shangqiu Medical College, Shangqiu 476100, China;
2. Zhengzhou Children's Hospital, Zhengzhou 450052, China |
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| Abstract: | AIM: To investigate the effect of Hedgehog (Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS: Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the HeLa cells was determined by RT-PCR and Western blot, respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS: Compared with control group, the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h (P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G0/G1 phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION: Smo gene silencing effectively inhibits the cell growth and induces the apoptosis of human cervical carcinoma cells. |
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| Keywords: | Smo gene Cervical carcinoma RNA interference Apoptosis |
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