Isolation and Identification of E. coli in the Microbial-fermentation Bed of Piggery |
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| Authors: | CHEN Qian-qian LIU Bo WANG Jie-ping LIU Guo-hong CHE Jian-mei CHEN Zheng TANG Jian-yang |
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| Institution: | 1. Agrobiological Resource Research Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350003, China;
2. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | To investigate the distribution and characteristic of E. coli in the microbial-fermentation bed of piggery, litters with different levels of fermentation were used for the isolation, identification and drug sensitivity test of E. coli, to analyze the pathogenicity and drug susceptibility of E. coli in the microbial-fermentation bed of piggery. The results showed that, E. coli could be isolated only from litters with low degree of fermentation (0<△E≤7.63), and totally 41 strains were isolated from litters with 10-5 dilution, but none from litters with higher degree of fermentation (△E>7.63). The number of E. coli reduced along with the fermentation of litters. Among the 41 strains of E. coli, 15 were intestinal pathogenic strains, 9 of which had astA gene (22%), and 2 strains had sepA gene (4.8%). 11 strains were resistant to doxycycline, accounted for 27% of the total.The two strains containing sepA gene had strong resistance to doxycycline. In conclusion, the microbial-fermentation bed fermentation bed had a inhibitory effect on E. coli which promoted the application of fermentation bed and reusing of litters. |
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| Keywords: | E coli serotype identification susceptibility test astA gene sepA gene |
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