The Study of Cell Wall Breaking Methods to Obtain Maximum Extraction Rate of Yeast Polysaccharides |
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| Authors: | LI Jing-jing JI Guo-zhen GAO Ai-wu CAO Ping YANG Jin-li WANG Hai-rong |
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| Institution: | 1. Laboratory of Animal Nutrition, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. College of Food Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
3. Key Laboratory of Dairy, Inner Mongolia Agricultural University, Hohhot 010018, China |
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| Abstract: | The aim of this study was to determine the optimal method of the yeast cell wall breaking which could obtain the maximum extraction rate of yeast polysaccharide. The extraction rate of yeast polysaccharide and the cell wall broken rate were used as the evaluating indexes. The conditions of ultrasonic disruption, repeated freezing and thawing, ultrasonic cleaning and protease treatment were optimized to select the appropriate parameters in cell wall breaking. The effects of these 4 methods and the synergistic action of ultrasonic disruption and protease treatment were compared. The results showed that when the ultrasonic cleaning time was 40 min and ultrasonic disruption time was 50 min, the water content of slurry was 15% and the times of freezing and thawing was 3, and the protease treatment was carried out at 40℃ for 15 h with enzyme concentration 300 IU/g and pH 5.0, respectively, the extraction rates of yeast polysaccharides were the highest. The effect of protease treatment was the best among these 4 cell wall breaking methods, its extraction rate of polysaccharide was 149.81 mg/g and the cell wall broken rate was 53.49%. The effect of combined way was better than that of single treatment, but the synergistic action would destroy the integrity of polysaccharide's structure. So the protease treatment was thought an ideal cell wall breaking method of yeast. |
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| Keywords: | yeast cell wall breaking methods polysaccharide extraction rate cell wall broken rate |
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