Isolation and Identification of Six Strains of Swine Bacillus and Their Probiotic Research |
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| Authors: | ZHONG Luo-hua DENG Shun-zhou ZHANG Wen-bo GAO Yang-gen XUE Rui LUO Feng WANG Jian-zhong |
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| Institution: | 1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
2. Nanchang Jinmu Animal Health Services Ltd., Nanchang 330013, China |
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| Abstract: | This study was aimed to separate Bacillus from healthy pig feces by high temperature treatment method and conventional anaerobic bacteria isolated method, and through the cultivation of traits, biochemical tests, PCR, bowel tolerance, antibacterial activity and vaccinated animals and other screening methods to identify stable properties and probiotic Bacillus. As a result, a total of six strains of Bacillus, which were one strain of huge Bacillus, one strain of Death Valley Bacillus, two strains of Bacillus amyloliquefaciens and two strains of Bacillus subtilis. Isolates were resistant to polymyxin and penicillin, were more sensitive to the other 12 kinds of commonly used drugs, and had better bacteriostatic action to pathogenic E.coli and Staphylococcus aureus. Four kinds of Bacillus were available under acidic, high temperature conditions and bile salts, in particular Bacillus megaterium and Bacillus subtilis were strong tolerance. Those isolated Bacillus were not pathogenic to mice, wherein Bacillus megaterium and Death Valley Bacillus had a more significant role in promoting the growth of animals. Finalized, the Bacillus megaterium and the Death Valley Bacillus could initially be as probiotic Bacillus choice. |
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| Keywords: | isolation and identification probiotic Bacillus |
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