Diagnosis of potato virus YN: a comparison between polyclonal and monoclonal antibodies and a biological assay |
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| Authors: | D G ROSE SUSAN McCARRA D H MITCHELL |
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| Institution: | Department of Agriculture and Fisheries for Scotland, Agricultural Scientific Services, East Craigs, Edinburgh EH12 8NJ, UK |
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| Abstract: | In an investigation conducted over 5 years, tests of samples from the Scottish Seed Potato Classification Scheme showed that when monoclonal antibodies (McABs) were used with the enzyme-linked immunosorbent assay (ELISA), they reliably detected the tobacco veinal necrosis strain of potato virus Y (PVYN). Polyclonal antibodies used in the first 3 years of the investigation were unreliable in differentiating PVYN from the ordinary strain (PVYN). It is suggested that ELISA and McABs be routinely used to diagnose PVYN and that all samples which give negative results be further assayed biologically to identify the virus or to indicate strains of PVYN not detectable by the McAB. Problems encountered with both assay systems are discussed. |
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