| PBP1基因在大肠杆菌中的表达及其表达产物的纯化 |
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| 引用本文： | 曾青兰,王志勇.PBP1基因在大肠杆菌中的表达及其表达产物的纯化[J].河南农业科学,2007(4):39-41. |
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| 作者姓名： | 曾青兰 王志勇 |
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| 作者单位： | 咸宁职业技术学院,湖北,咸宁,437000 |
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| 摘 要： | 将获得的拟南芥黑芥子酶结合蛋白PBP1基因表达载体pTYB2-PBP1导入大肠杆菌ER2566细胞,经IPTG诱导,SDS-PAGE电泳显示,PBP1-CBD融合蛋白获得了较好的表达。进一步对PBP1蛋白进行分离纯化,SDS-PAGE电泳结果显示分离纯化效果较好,所获得的纯化的PBP1蛋白可进一步用于研究PBP1与MBP6的酵母双杂交技术。
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| 关 键 词： | PBP1基因 大肠杆菌 融合蛋白 表达 纯化 |
| 文章编号： | 1004-3268(2007)04-0039-03 |
| 修稿时间： | 2007-01-12 |
Expression of PBP1 Gene in E.coli and Purification of Its Expressive Product |
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| ZENG Qing-lan,WANG Zhi-yong.Expression of PBP1 Gene in E.coli and Purification of Its Expressive Product[J].Journal of Henan Agricultural Sciences,2007(4):39-41. |
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| Authors: | ZENG Qing-lan WANG Zhi-yong |
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| Institution: | Xianning Vocational Technical College, Xianning 437000, China |
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| Abstract: | In order to obtain purified PBP1 protein to study the reciprocity between PBP1 protein and MBP6,the expressive vector pTYB2PBP1 of the PBP1 gene of myrosase-binding protein from Arabidopsis was transformed into E.coli ER2566.The results by SDS-PAGE showed that the fusion protein PBP1-CBD was expressed well by IPTG induction.The expressive product was separated and purified and the purified protein can be used to study the Yeast Two-Hybrid System technology between PBP1 and MBP6. |
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| Keywords: | PBP1 gene E coli Fusion protein Expression Purification |
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