| 嗜水气单胞菌粘附特性的研究 |
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| 引用本文： | 严亚贤,孙建和,陈怀青,陆承平.嗜水气单胞菌粘附特性的研究[J].中国兽医学报,1995(4). |
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| 作者姓名： | 严亚贤 孙建和 陈怀青 陆承平 |
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| 作者单位： | 南京农业大学动物医学院，上海农学院动物科学系 |
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| 摘 要： | 不同来源的６株嗜水气单胞菌（Ａｅｒｏｍｏｎａｓｈｙｄｒｏｐｈｉｌａ）对１０种不同红细胞的血凝试验表明，Ｊ－１株能凝集所有的１０种红细胞，而与其他菌株的血凝谱有所不同。血凝图式有２种：人、鸡、麻雀的红细胞凝集快，呈大小不等的絮状；而鲫鱼、绵羊、猪、小鼠、兔、鸭、犬的红细胞凝集慢，且呈均匀颗粒状。这两种血凝均能被Ｄ－甘露糖抑制，但不能被Ｊ－１株Ｒ菌毛抑制。组织粘附试验显示，Ｊ－１株能粘附小鼠和鲫鱼的肠组织和肠绒毛。将提纯的Ｒ菌毛预处理肠组织，或用Ｄ－甘露糖或木瓜蛋白酶消化的Ｒ菌毛抗血清Ｆａｂ片段预处理菌体，都不能抑制上述的粘附作用。Ｊ－１株对ＨＥｐ－２细胞显示强粘附，菌体随机粘附在细胞上，有少数侵入细胞浆内。其余５株菌的粘附特性与Ｊ－１株不尽相同。所有６株菌对草鱼肠组织及肠绒毛、ＥＰＣ细胞（鲤鱼乳头状上皮瘤细胞系）均无粘附性。
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| 关 键 词： | 嗜水气单胞菌，血凝谱，肠组织粘附，细胞粘附 |
Adhesion of Aeromonas hydrophila |
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| Yan Yaxian, Sun Jianhe, Chen Huaiqing, Lu Chengping.Adhesion of Aeromonas hydrophila[J].Chinese Journal of Veterinary Science,1995(4). |
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| Authors: | Yan Yaxian Sun Jianhe Chen Huaiqing Lu Chengping |
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| Abstract: | Aeromonas hydrophila J-1 agglutinated erythrocytes of human(O group ), chicken,sparrow,crucian carp, rabbit,dog,sheep, duck,mouse and pig and the agglutination were inhibitedby 1%D-mannose. The organism J-1 adhered to mouse, crucian carp intestines and HEp-2 cells,Theadhesion were not inhibited by pretreating the intestines with purified R pili or 1%D-mannose or bypretreating the organisms with Fab fraction of R pili antibody.The pellicle formation and autoaggrega-tion of the organisms could be inhibited by the antipili Fab.The purified R pili did not adhere to the in-testines,Hep-2 cells or the erythrocytes.The other 5 Aeromonas hydrophila strains showed differentcharacteristics. The highly adherent strains also produced virulent extracellular toxin and showed highlypathogenic. |
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| Keywords: | Aeromonas hydrophila hemagglutination intestine adhesion cell adhesion |
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