| 棉花黄萎病菌插入突变体库的构建及致病相关基因DVK1的克隆与鉴定 |
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| 引用本文： | 高峰,彭姗,彭晓玲,李晖,李国英.棉花黄萎病菌插入突变体库的构建及致病相关基因DVK1的克隆与鉴定[J].棉花学报,2011,23(1):64-68. |
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| 作者姓名： | 高峰 彭姗 彭晓玲 李晖 李国英 |
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| 作者单位： | 1. 石河子大学绿洲农作物病害防控重点实验室,石河子大学,新疆,石河子,832003
2. 中国农业大学植物病理系,北京,100193 |
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| 基金项目： | 农业部基础性工作专项(SB2007FY027) |
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| 摘 要： | 利用ATMT（Agrobacterium tumefaciens-mediated transformation）技术自建Verticillium dahliae强致病力落叶型菌株T-DNA插入突变体库，共获得5000个转化子；随机挑选1000个转化子进行致病力鉴定，筛选获得5株致病力衰退的突变体。挑选致病力下降最为明显的突变体d1，通过TAIL-PCR技术最终获得一段长3237 bp的DVK1全长cDNA序列，编码1079个氨基酸的蛋白。基于DNA序列比对发现，DVK1基因含有2个内含子，分别为52 bp和36 bp。进一步比对发现T-DNA插入到DVK1基因启始密码上游740 bp的启动子中。通过遗传互补实验d1 恢复了致病性，进一步证明DVK1基因与黄萎菌致病性相关。
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| 关 键 词： | T-DNA插入 突变体文库 基因克隆 棉花黄萎病 功能鉴定 |
| 收稿时间： | 2010-06-11; |
Identification of the Pathogenicity-related Gone DVK1 in Verticillium dahliae |
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| GAO Feng,PENG Shan,PENG Xiao-ling,LI Hui,LI Guo-ying.Identification of the Pathogenicity-related Gone DVK1 in Verticillium dahliae[J].Cotton Science,2011,23(1):64-68. |
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| Authors: | GAO Feng PENG Shan PENG Xiao-ling LI Hui LI Guo-ying |
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| Institution: | GAO Feng1,PENG Shan1,PENG Xiao-ling1,LI Hui2,LI Guo-ying1 (1.The Key Laboratory of Prevention and Control for Oasis Crop Disease,Shihezi University,Shihezi,Xinjiang 832003,China,2.Department of Plant Pathology,China Agricultural University,Beijing 100193,China) |
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| Abstract: | In this study,we carried out T-DNA insertional mutagenesis to identify mutants,which are effective in pathogenicity.The highly virulent,defoliating strain was mutated through Agrobacterium tumefaciens-mediated transformation,and 5000 transformants were obtained.Pathogenicity test of randomly selected 1000 transformants found that five mutants lost their virulence on a susceptible cotton cultivar.A new mutant,d1,was unable to cause full disease on cotton.Analysis of the mutation using Thermal Asymmetric Inte... |
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| Keywords: | T-DNA insertional mutants library gene clone Verticillium dahliae functional identification |
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