Spread of potato virus Y in potato cultivars (Solanum tuberosum L.) with different levels of sensitivity |
| |
| Authors: | Nata&#x;a Mehle Maja Kova
Nata&#x;a Petrovi
Maru&#x;a Pompe Novak &#x;pela Baebler Hana Kre
i
Stres Kristina Gruden Maja Ravnikar |
| |
| Institution: | Department of Plant Physiology and Biotechnology, National Institute of Biology, Večna pot 111, 1000 Ljubljana, Slovenia |
| |
| Abstract: | The aim of this work was to correlate the appearance of the symptoms, multiplication and spread of virus after mechanical inoculation of potato (Solanum tuberosum L.) cultivars showing different levels of susceptibility and sensitivity to Potato virus YNTN (PVYNTN). The potato cultivars used were the resistant cultivar Sante and susceptible cultivars Igor, Pentland squire and Désirée. The spread of the virus PVYNTN in infected plants was monitored using different methods: DAS-ELISA, tissue printing, immuno-serological electron microscopy and real-time PCR. In all three susceptible cultivars, the virus was detected in the inoculated leaves 4–5 days after inoculation. From there virus spread rapidly, first into the stem, then more or less simultaneously to the upper leaves and roots. Real-time PCR was shown to be very sensitive and enabled viral RNA to be detected in non-inoculated leaves of susceptible cultivar Igor earlier than other methods. Therefore, for exact studies of plant–virus interaction, a combination of methods which detect viruses on the basis of their different properties (coat protein, morphology or RNA) should be used to monitor the spread of viruses. |
| |
| Keywords: | Solanum tuberosum L Virus spread PVYNTN DAS-ELISA Tissue printing Immuno-serological electron microscopy Real-time PCR |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
