| 铝胁迫下大豆根系脱落酸累积与柠檬酸分泌的关系研究 |
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| 引用本文： | 尤江峰,候宁宁,徐睦芸,刘宁,张红梅,兰图,杨振明.铝胁迫下大豆根系脱落酸累积与柠檬酸分泌的关系研究[J].植物营养与肥料学报,2010,16(4):899-905. |
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| 作者姓名： | 尤江峰 候宁宁 徐睦芸 刘宁 张红梅 兰图 杨振明 |
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| 作者单位： | 1.吉林大学植物科学学院,吉林大学农学部土壤-植物分子遗传学实验室,吉林长春 130062 |
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| 基金项目： | 国家自然科学基金，教育部博士点新教师基金 |
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| 摘 要： | 为探讨铝(Al)胁迫条件下脱落酸(ABA)调控植物根系有机酸分泌的机制,进行了ABA与Al诱导大豆根系柠檬酸分泌的关系试验。结果表明:1)外源ABA和ABA合成抑制剂fluridone分别提高和降低了Al诱导的大豆根尖ABA含量的增加,但对根系柠檬酸分泌量均无影响,ABA对根系内源柠檬酸含量和柠檬酸合成酶的活性也没有影响;2)分根试验表明,与Al直接接触的根部(Part A)内源ABA含量发生变化,且有柠檬酸的分泌,而不与Al直接接触的根部(Part B)内源ABA含量也发生变化,但没有柠檬酸分泌;3)Al胁迫下,大豆耐Al基因型柠檬酸分泌量远高于敏感基因型,但二者的内源ABA含量却没有差异;4)30μmol AlCl3处理,在0~12 h柠檬酸分泌速率和内源ABA含量随Al处理时间增加而增加,去除Al胁迫时(12~18 h),柠檬酸分泌速率继续增加,但内源ABA含量则迅速下降。综合以上结果,推测ABA不是通过提高Al诱导柠檬酸分泌来调控大豆耐Al性。
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| 关 键 词： | 铝胁迫 大豆 柠檬酸 脱落酸 |
| 收稿时间： | 2009-9-16 |
Study on the relationship of abscicic acid accumulation and citrate secretion of soybean roots under aluminum stress |
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| YOU Jiang-feng,HOU Ning-ning,XU Mu-yun,LIU Ning,ZHANG Hong-mei,LAN Tu,YANG Zhen-ming.Study on the relationship of abscicic acid accumulation and citrate secretion of soybean roots under aluminum stress[J].Plant Nutrition and Fertilizer Science,2010,16(4):899-905. |
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| Authors: | YOU Jiang-feng HOU Ning-ning XU Mu-yun LIU Ning ZHANG Hong-mei LAN Tu YANG Zhen-ming |
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| Institution: | 1.College of Plant Science,Jilin University/Laboratory of Soil-Plant Molecular Genetics,Agronomy Division,Jilin University,Changchun,China 130062 |
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| Abstract: | Aluminum (Al)-induced organic acid anions secretion is known as one of important Al-resistance mechanisms in plants. Abscisic acid (ABA) plays an important role in mediating some biotic and abiotic stresses. A series of experiments including split-root experiment and time course experiment were carried out to clarify whether ABA is involved in the process of Al-induced secretion of citrate from soybean roots. The results are as follows: 1) ABA accumulation in Al-induced soybean roots is increased under the exogenous ABA application, while ABA accumulation is inhibited under the Fluridone (ABA biosynthesis inhibitor). However, the both applications no effects on either Al induced citrate exudation, or the Al induced increase of citrate content and citrate synthase activity. 2) In the split-root experiment (the whole roots of soybean plant were separated equally to Part A and Part B), endogenous ABA content of root apices is increased in part A, while the content is decreased in part B when roots of part A are exposed to Al (Part A, +Al; Part B, -Al), and Al-induced citrate secretion is only found in part A. 3) The amount of citrate secretion of Al-tolerant cultivar is higher than that of Al-sensitive cultivar, while the ABA contents of roots of both cultivars have no differences under same Al stress. 4) In the time course experiment, both citrate secretion rate and endogenous ABA content are increased with increase of Al treatment time during 0-12 h. During the next 6 h pulse (12-18 h) without Al stress, endogenous ABA is dropped quickly, while citrate secretion rate is increased. The above results suggest that endogenous ABA in soybean roots is not involved in modulating Al-induced citrate exudation. |
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| Keywords: | Al stress soybean citrate abscicic acid |
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