| ‘新高’梨花粉败育与内源多胺含量变化的关系 |
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| 引用本文： | 李六林,张绍铃,郭艳玲.‘新高’梨花粉败育与内源多胺含量变化的关系[J].园艺学报,2007,34(2):301-304. |
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| 作者姓名： | 李六林 张绍铃 郭艳玲 |
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| 作者单位： | (南京农业大学园艺学院, 南京210095;山西农业大学园艺学院, 山西太谷030801) |
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| 摘 要： | 以花粉败育的‘新高’梨和花粉可育的‘丰水’梨为试材, 对不同发育阶段的花蕾或花药内不同形态的腐胺( Put) 、亚精胺( Spd) 和精胺( Spm) 含量进行了分析, 以探讨多胺种类和形态与新高花粉败育的关系。结果表明: 新高花蕾发育过程中游离态的Put和Spd含量变化幅度不大, 丰水则在单核花粉到二核花粉时期出现高峰, 在单核花粉期到单核花粉晚期含量是新高的3～5倍。新高中游离态的Spm也显著低于丰水; 丰水中高氯酸可溶共价结合态Put、Spd和Spm含量逐渐增加, 新高在花粉败育前期或败育期出现不同于丰水的高峰; 除丰水成熟的花药外, 两个品种中高氯酸不溶结合态多胺含量的变化基本一致; 两个品种的不同形态Put、Spd和Spm总量在败育前期差异不显著, 此后新高显著低于丰水; 丰水中的多胺均以游离态为主, 新高以高氯酸可溶结合态为主。
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| 关 键 词： | 梨 花粉 败育 多胺 |
| 文章编号： | 0513-353X（2007）02-0301-04 |
| 收稿时间： | 2006-06-07 |
| 修稿时间： | 2006-06-072007-02-13 |
Relationship Between Pollen Abortion and Change of Endogenous Polyamines Contents in 'Niitaka' Pear Cultivars |
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| LI Liu-lin,ZHANG Shao-ling,GUO Yan-ling.Relationship Between Pollen Abortion and Change of Endogenous Polyamines Contents in ''''Niitaka'''' Pear Cultivars[J].Acta Horticulturae Sinica,2007,34(2):301-304. |
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| Authors: | LI Liu-lin ZHANG Shao-ling GUO Yan-ling |
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| Institution: | (College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801, China) |
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| Abstract: | In order to explore the relationship between polyamines and pollen abortion of pear,the contents of different forms of putrescine(Put),spermidine(Spd)and spermine(Spm)were examined at different stages of pollen development in floral buds or anther of male sterile pear cultivar Niitaka(Pyrus pyrifolia Nakai)and fertile pear cultivar Housui.The results indicated that the variation range of contents of both free Put and Spd in Niitaka was small during bud development,whereas the variation of free Put and Spd content of Housui showed a peak during uninucleate pollen stage to later uninucleate pollen stage.At the stage of pollen abortion(from uninucleate pollen stage to later uninucleate pollen stage),the free Put and Spd contents were 3-5 times of Niitaka.Free Spm content in Niitaka was significantly lower than that of Housui.The contents of perchloric acid covalently soluble conjugated Put,Spd and Spm of Housui were gradually increased,but the contents of those in Niitaka showed a peak that did not appear in Housui during pollen abortion or ahead.Except developmentally mature anther,variations of perchloric acid covalently insoluble conjugated in two cultivars were similar.Total contents of different forms of Put,Spd and Spm did not have significant differences before pollen abortion,but were significant lower in Niitaka than that of Housui after pollen abortion.The free Put,Spd and Spm were the predominant forms in total contents in Housui,however,the predominant forms in Niitaka were perchloric acid covalently soluble conjugated Put,Spd and Spm. |
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| Keywords: | Pear Pollen Abortion Polyamines |
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