| 玫瑰基因组AFLP反应体系的建立和优化 |
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| 引用本文： | 李玉舒,张启翔.玫瑰基因组AFLP反应体系的建立和优化[J].北京农业职业学院学报,2008,22(5). |
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| 作者姓名： | 李玉舒 张启翔 |
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| 作者单位： | 北京农业职业学院,北京,102442;北京林业大学,北京,100083 |
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| 基金项目： | "十五"国家科技攻关资助项目 |
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| 摘 要： | 以玫瑰(Rosa rugosa)叶片为材料,利用改良的CTAB法,提取到了高质量的玫瑰叶片DNA,并建立了37个玫瑰品种和5个蔷薇品种的AFLP银染反应体系,首次在玫瑰DNA提取、酶切连接、预扩增、选择性扩增等实验过程取得了成功,得到了清晰的玫瑰品种的AFLP指纹图谱,为玫瑰基因库的建立、优良品种选育以及亲缘关系的系列研究奠定了理论基础.
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| 关 键 词： | 玫瑰 DNA CTAB法 AFLP 指纹图谱 |
DNA Template Preparation and Technology System Building for AFLP Analysis of Rosa Rugosa. |
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| Li Yushu,Zhang Qixiang.DNA Template Preparation and Technology System Building for AFLP Analysis of Rosa Rugosa.[J].Journal of Beijing Agricultural Vocation College,2008,22(5). |
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| Authors: | Li Yushu Zhang Qixiang |
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| Institution: | Li Yushu1,Zhang Qixiang2(1.Beijing Agricultural Vocation College,Beijing 102442,China,2.Beijing Forestry University,Beijing 100083,China) |
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| Abstract: | Extraction of DNA with phenol-cholorofore method from Rosa rugosa leaves was dealt with and AFLP finger printing in 37 R.rugosa cultures and 5 Rosa davurica were established.Clear and high resolution DNA finger prints were obtained,which mena success in DNA extraction,restriction-ligasereaction,pre-amplification and staining techniques.A foundation for the establishment of gene pool,selection and breeding of eminent cultivars and geneic relaionshpi in R.rugosa was laid. |
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| Keywords: | DNA AFLP |
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