| 加工番茄遗传转化再生体系的建立 |
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| 引用本文： | 张录霞,郝青南,马超,段小瑜,牛建新,马兵钢.加工番茄遗传转化再生体系的建立[J].西北农业学报,2008,17(3):236-241. |
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| 作者姓名： | 张录霞 郝青南 马超 段小瑜 牛建新 马兵钢 |
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| 作者单位： | 石河子大学农学院园艺系,新疆,832000 |
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| 基金项目： | 国家自然科学基金
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新疆自治区高校科研计划 |
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| 摘 要： | 加工番茄种子出芽后7~8 d,子叶剪成约0.2~0.4 cm,0.3~0.5 cm的小块,以MS B5为基本培养基,附加IAA0.2 mg/L分别与0.5,1.0,1.5,2.0,2.5 mg/L的6-BA/ZT/TDZ组合;6-BA2.0 mg/L分别与0.0,0.05,0.1,0.2,0.5,1.0 mg/L的IAA/IBA/NAA组合。经诱芽比较,在不同浓度6-BA/ZT/TDZ与0.2mg/LIAA组合中,出芽率最高的培养基为MS ZT1.0 mg/L IAA0.2 mg/L和MS TDZ2.0 mg/L IAA0.2 mg/L。在不同浓度的IAA/IBA/NAA与6-BA2.0 mg/L组合中,IAA明显优于NAA和IBA,筛选出MS 2.0 mg/L6-BA 1.0 mg/LIAA为最佳生芽培养基。以1/2MS添加NAA//BA0.0,0.1,0.2,0.5,1.0 mg/L进行生根比较,再生芽在MS 0.5 mg/L IBA生根培养基上生根最好,并发育成完整的小植株。
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| 关 键 词： | 加工番茄 遗传转化 再生体系 子叶 |
| 收稿时间： | 2007/12/24 0:00:00 |
| 修稿时间： | 2008/1/5 0:00:00 |
Processing Tomato Plant Regeneration in Genetic Transformation |
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| ZHANG Lu-xi,HAO Qing-nan,MA Chao,DUAN Xiao-yu,NIU Jian-xin and MA Bing-gang.Processing Tomato Plant Regeneration in Genetic Transformation[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(3):236-241. |
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| Authors: | ZHANG Lu-xi HAO Qing-nan MA Chao DUAN Xiao-yu NIU Jian-xin and MA Bing-gang |
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| Institution: | Department of Horticulture, Agricultural College, Shihezi University, Xinjiang 832000, China;Department of Horticulture, Agricultural College, Shihezi University, Xinjiang 832000, China;Department of Horticulture, Agricultural College, Shihezi University, Xinjiang 832000, China;Department of Horticulture, Agricultural College, Shihezi University, Xinjiang 832000, China;Department of Horticulture, Agricultural College, Shihezi University, Xinjiang 832000, China;Department of Horticulture, Agricultural College, Shihezi University, Xinjiang 832000, China |
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| Abstract: | When tomato seedlings grew 7~8 days after germination cotyledons were cut into 0.2~0.4,0.3~0.5 cm discs as explants.MS B5 was used as basic medium,and IAA0.2 mg/L combined with different concentrations of 6-BA/ZT/TDZ on bud induction;6-BA2.0 mg/L combined with different concentrations of IAA /IBA/NAA on bud induction.The results showed that the best shoot induction medium for cotyledon explants was MS ZT1.0 mg/L IAA0.2 mg/L and MS TDZ2.0 mg/L IAA0.2 mg/L in different combinations of IAA0.2 mg/L and 6-BA/ZT/TDZ.Among different combinations of 6-BA2.0 mg/L and IAA/IBA/NAA,IAA was bester than that of IAA and IBA on bud induction.The best medium on bud induction was MS 6-BA 2.0 mg/ L IAA 1.0 mg/ L.The root could be induced after the regenerated buds were planted in M S IBA 0.5 mg/L one week later. |
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| Keywords: | Processing tomato Genetic transformation Regeneration system Cotyledon |
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