| 核桃RAPD引物筛选及品种间亲缘关系研究 |
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| 引用本文： | 张美勇,上官凌飞,徐颖,相昆,房经贵.核桃RAPD引物筛选及品种间亲缘关系研究[J].中国农学通报,2010,26(2):84-90. |
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| 作者姓名： | 张美勇 上官凌飞 徐颖 相昆 房经贵 |
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| 作者单位： | 1. 山东省果树研究所,山东泰安,271000
2. 南京农业大学园艺学院,南京,210095 |
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| 基金项目： | 国家科技支撑计划资助项目"农林动植物育种工程" |
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| 摘 要： | 以30个核桃品种为试验材料,首次建立了11 nt引物长度的优化核桃RAPD技术体系。从40个11 bp碱基中共筛选出适用于核桃遗传多样性分析18个11 nt引物。这些引物在30个核桃品种中共扩增出111个DNA片段,片段大小在500~3050之间,其中77个DNA片段表现出多态性,占总扩增片段的69.4%。根据RAPD指纹信息对核桃品种资源的遗传距离进行了分析,并据此构建了聚类树状图。从聚类结果看,30个核桃品种可以划分成遗传差异较明显的10组。
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| 关 键 词： | 云南省 云南省 设施园艺 发展 对策 |
| 收稿时间： | 2009-08-25 |
| 修稿时间： | 2009-09-16 |
Screening of RAPD Primer for Study of Genetic Relationship among Walnut(Juglans regia L.) Cultivars |
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| Zhang Meiyong,Shangguan Lingfei,Xu Ying,Xiang Kun,Fang Jinggui.Screening of RAPD Primer for Study of Genetic Relationship among Walnut(Juglans regia L.) Cultivars[J].Chinese Agricultural Science Bulletin,2010,26(2):84-90. |
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| Authors: | Zhang Meiyong Shangguan Lingfei Xu Ying Xiang Kun Fang Jinggui |
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| Institution: | Zhang Meiyong1,Shangguan Lingfei2,Xu Ying1,Xiang Kun1,Fang Jinggui2(1Sh,ong Institute of Pomology,Tai\'an Sh,ong 271000,2College of Horticulture,Nanjing Agricultural University,Nanjing 210095) |
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| Abstract: | In this study,the preferential RAPD technology for walnut genetic analysis was successfully developed with utilization of 11 nt length primers instead of 10 nt ones and accurate selection of annealing temperatures,which was the results from the study on 30 walnut cultivars.Based on RAPD fingerprint quality,eighteen 11nt-primers have been screened from 40 ones for further walnut genetic analysis.These 18 primers could amplify 111 PCR bands ranging from 500 to 3050 bp in size,among which 77 were polymorphic b... |
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| Keywords: | RAPD |
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