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| 油茶愈伤组织和芽诱导培养条件的筛选 | |
| 引用本文： | 黄莉雅,张日清,马锦林,潘晓芳,陈国臣,何荣飞,伍有样.油茶愈伤组织和芽诱导培养条件的筛选[J].经济林研究,2010,28(1):30-34. |
| 作者姓名： | 黄莉雅 张日清 马锦林 潘晓芳 陈国臣 何荣飞 伍有样 |
| 作者单位： | 1. 中南林业科技大学,林学院,湖南长沙410004 2. 广西林业科学研究院,广西南宁,530002 3. 广西大学林学院,广西南宁,530001 |
| 基金项目： | 林业科技支撑计划专题“广西高产优秀油茶新品种选育”(2006BAD18B02-04) |
| 摘 要： | 为了筛选出较为适宜的油茶愈伤组织和芽诱导的培养条件,以优良无性系岑软2号和岑软3号鲜嫩带芽的茎段为试验材料,初步研究了不同外植体消毒方式、培养基蔗糖和琼脂含量、试验用水、培养基类型、采样时间及是否光照培养等不同的培养条件对油茶愈伤组织和芽的诱导的影响。研究结果表明:7月份所采外植体的愈伤组织和芽的诱导率高于其它月份;以MS为基本培养基、采用蒸馏水配制培养基、蔗糖质量浓度为30g/L、琼脂质量分数0.51%为最佳油茶愈伤组织和芽诱导培养基;以0.1%HgCl2对外植体处理6min的消毒效果最好;接种后直接进行光照培养效果较好。 |
| 关 键 词： | 油茶 组织培养 培养条件 研究 |
Screening of induction conditions of callus and bud in Camellia oleifera |
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| HUANG Li-ya,ZHANG Ri-qing,MA Jin-lin,PAN Xiao-fang,CHEN Guo-chen,HE Rong-fei,WU You-xiang.Screening of induction conditions of callus and bud in Camellia oleifera[J].Economic Forest Researches,2010,28(1):30-34. | |
| Authors: | HUANG Li-ya ZHANG Ri-qing MA Jin-lin PAN Xiao-fang CHEN Guo-chen HE Rong-fei WU You-xiang |
| Institution: | 1.Forestry College;Central South University of Forestry & Technology;Changsha 410004;Hunan;China;2.Guangxi Academy of Forestry;Nanning 530002;Guangxi;3. Forestry College;Guangxi University;Nanning 530001;China |
| Abstract: | In order to find suitable induction conditions of callus and bud in Camellia oleifera, taking stem with fresh buds from Cenruan No.2 and Cenruan No.3 in C. oleifera as explants, effects of some conditions on induction of callus and bud in C. oleifera were researched, for example, different disinfection ways of explants, concentrations of sucrose and agar, test water, kinds of medium, explants sampling time, illumination condition, and so on. The results showed that July was the best explants sampling time, ... |
| Keywords: | Camellia oleifera tissue culture culture conditions study |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |