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| 猪不同直径卵泡中卵母细胞体外培养的成熟潜力 | |
| 引用本文： | 韩毅冰,施维民,秦鹏春.猪不同直径卵泡中卵母细胞体外培养的成熟潜力[J].中国兽医学报,2000,20(2):183-187. |
| 作者姓名： | 韩毅冰 施维民 秦鹏春 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 2. 黑龙江省家畜繁育指导站,黑龙江,哈尔滨,150000 3. 东北农业大学,生物工程系,黑龙江,哈尔滨,150030 |
| 摘 要： | 比较研究了猪卵巢表面直径０．５～１ｍｍ、１～２ｍｍ、２～６ｍｍ、〉６ｍｍ卵泡中卵母细胞所处的发生泡阶段。结果表明，０．５～１ｍｍ卵泡中卵母细胞主要处于ＧＶ－Ｉ用ＧＶ－Ⅱ期２个阶段，比例分别为６７．２％和２０．６％；而１～２ｍｍ和２～６ｍｍ直径卵泡中，卵母细胞大部分则处于ＧＶ－Ⅱ期，比例分别为９１．９％和８９．６％；〉６ｍｍ直径卵泡中，卵母细胞逐渐发生老化，ＧＶ－Ⅱ期比例仅为５３．２％，一部分处于Ｖ |
| 关 键 词： | 卵泡 卵母细胞 减数分裂 体外成熟 dbcAMP 猪 |
| 修稿时间： | 1998-10-30 |
Maturation Competence of the Pig Oocytes from Different Diameters of Follicles |
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| HAN Yi-bing,SHI Wei-min,Qin Peng-chun.Maturation Competence of the Pig Oocytes from Different Diameters of Follicles[J].Chinese Journal of Veterinary Science,2000,20(2):183-187. | |
| Authors: | HAN Yi-bing SHI Wei-min Qin Peng-chun |
| Abstract: | The stages of oocytes germinal vesicles from fol l icles in diameters of 0.5-1 mm, 1-2 mm, 2-6 mm and bigger than 6 mm were comp are d in this experiment. Oocytes from 0.5-1 mm follicles were mainly at GV-Ⅰ and GV-Ⅱ, with proportions of 67.2% and 20.6% respectively. Whereas most of the oo cytes from 1-2 mm(91.9%) and 2-6 mm(89.6%) were at GV-Ⅱ. 53.2% of the oocyte s from follicles bigger than 6 mm were at GV-Ⅱ, the others were at GV-Ⅳ and GV BD, indicative of their aging. In vitro maturation indicated that oocytes from 1-2 mm follicles had a l ower maturation rate than that from 2-6 mm follicles. Among them, 25.1% couldn ′ t go to GVBD, 39.8% stopped at M-Ⅰ. Addition of 1 mmol/L dbcAMP from 0-20 h o f culture could effectively inhibit GVBD. Removal of dbcAMP in the following stag es increased the maturation rate from both follicles and decreased the percentag e of oocytes arrested at GV stage, but didn′t affect those arrested at M-Ⅰ. S o, the effect of dbcAMP may mainly act for synchronization of GVBD. |
| Keywords: | follicles oocytes meiosis in vitro maturation dbcAMP |
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