| 白沙蒿肌动蛋白基因核心片段的克隆和序列分析 |
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| 引用本文： | 张一弓,张丽静,刘永红,傅华.白沙蒿肌动蛋白基因核心片段的克隆和序列分析[J].广西农业生物科学,2009(2):251-254. |
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| 作者姓名： | 张一弓 张丽静 刘永红 傅华 |
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| 作者单位： | 农业部草地农业生态系统学重点开放实验室;兰州大学草地农业科技学院; |
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| 基金项目： | 国家重点研究发展计划973(2007CB108903);;国家自然科学基金(30800801)资助 |
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| 摘 要： | 本研究以荒漠植物白沙蒿总RNA为模板，运用RT-PCR方法扩增出肌动蛋白基因核心序列。将获得的片段克隆到T载体后进行测序，序列分析表明：白沙蒿肌动蛋白基因核心片段长599bp，编码198个氨基酸。将该序列在GenBank中注册，并与多种植物肌动蛋白序列进行同源性比较，发现该片段的核酸序列同源性在75％以上，氨基酸序列同源性在85％以上，具有高度保守性。
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| 关 键 词： | 白沙蒿 肌动蛋白基因 基因核心片段 序列分析 |
Cloning and Sequence Analysis of Actin Gene Fragment from Artemisia sphaerocephala krasch |
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| Zhang Yigong Zhang Lijing Liu Yonghong Fu Hua Key Laboratory of Grassl, Agro-ecosystem,Ministry of Agriculture,College of Pastoral Agriculture Science , Technology,Lanzhou University,Lanzhou.Cloning and Sequence Analysis of Actin Gene Fragment from Artemisia sphaerocephala krasch[J].Journal of Guangxi Agricultural and Biological Science,2009(2):251-254. |
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| Authors: | Zhang Yigong Zhang Lijing Liu Yonghong Fu Hua Key Laboratory of Grassl Agro-ecosystem Ministry of Agriculture College of Pastoral Agriculture Science Technology Lanzhou University Lanzhou |
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| Institution: | Zhang Yigong Zhang Lijing Liu Yonghong Fu Hua Key Laboratory of Grassl, Agro-ecosystem,Ministry of Agriculture,College of Pastoral Agriculture Science , Technology,Lanzhou University,Lanzhou,730020 |
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| Abstract: | In this study,total RNA was extracted from Artemisia sphaerocephala krasch. The Actin gene fragment was obtained by RT-PCR and was sequenced after cloning into pUCm-T vector. The result revealed that it con-tains 599 bp,encoding a protein of 198 amino acid. Similarity comparison with other actin gene sequence in the GenBank shows it shares over 75% nucleotide sequence similarity and over 85% amino acid sequence similarity with other actin,which suggests that the Artemisia sphaerocephala krasch Actin gene is... |
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| Keywords: | Artemisia sphaerocephala krasch Actin gene Gene fragment Sequence analysis |
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