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| NO和H2O2在IBA诱导万寿菊不定根形成中的作用 | |
| 引用本文： | 廖伟彪,黄高宝,郁继华,张美玲.NO和H2O2在IBA诱导万寿菊不定根形成中的作用[J].园艺学报,2011,38(5):939-946. |
| 作者姓名： | 廖伟彪 黄高宝 郁继华 张美玲 |
| 作者单位： | 1. 甘肃省干旱生境作物学重点实验室,甘肃农业大学农学院,兰州,730070;中国科学院寒区旱区环境与工程研究所,兰州,730020 2. 甘肃省干旱生境作物学重点实验室,甘肃农业大学农学院,兰州,730070 3. 甘肃农业大学理学院,兰州,730070 |
| 基金项目： | 中国博士后科学基金，教育部科学技术研究重点项目，甘肃省科技厅重大专项计划，甘肃省自然科学基金，甘肃省干旱生境作物学重点实验室开放基金课题 |
| 摘 要： | 研究了一氧化氮（NO）和过氧化氢（H2O2）在吲哚丁酸（IBA）诱导万寿菊（Tagetes erecta L.）外植体不定根形成过程中的作用及其相互关系。结果表明：外源IBA与NO、H2O2一样对万寿菊外植体不定根形成有促进作用，且IBA与NO，IBA与H2O2协同增效。NO清除剂cPTIO和H2O2清除剂CAT均能抑制IBA对不定根形成的促进作用。同样，cPTIO和CAT亦能抵消IBA对NPA抑制不定根形成的逆转作用。可见，NO和H2O2是IBA诱导万寿菊不定根形成的必要条件。IBA处理提高了外植体内源NO和H2O2的含量，说明IBA是通过增加内源NO和H2O2水平实现对不定根形成的促进作用。cPTIO和L-NAME抑制了IBA对H2O2含量的促进作用，而CAT和DPI却不能抑制IBA对NO含量的促进作用。可见，NO和H2O2是IBA诱导万寿菊不定根形成的两个下游信号分子，且NO可能位于H2O2的上游。 |
| 关 键 词： | 万寿菊 Tagetes erecta L. 一氧化氮 过氧化氢 吲哚丁酸 不定根 |
Nitric Oxide and Hydrogen Peroxide are Involved in Indole-3-butyric Acid-induced Adventitious Root Formation of Marigold |
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| LIAO Wei-biao,HUANG Gao-bao,YU Ji-hua,ZHANG Mei-ling.Nitric Oxide and Hydrogen Peroxide are Involved in Indole-3-butyric Acid-induced Adventitious Root Formation of Marigold[J].Acta Horticulturae Sinica,2011,38(5):939-946. | |
| Authors: | LIAO Wei-biao HUANG Gao-bao YU Ji-hua ZHANG Mei-ling |
| Institution: | LIAO Wei-biao1,2,HUANG Gao-bao1,YU Ji-hua1,and ZHANG Mei-ling3 (1Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement,College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China,2Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Lanzhou 730020,3College of Science,China) |
| Abstract: | The roles of NO and H2O2 in indole-3-butyric acid(IBA)-induced adventitious root formation in marigold plants(Tagetes erecta L.)and the order of action of these molecules within the signal transduction pathway were investigated. IBA treatment was able to induce de novo root organogenesis in marigold explants mimicking the effects of NO and H2O2. Treatment of IBA plus NO or H2O2 promoted adventitious root development compared with explants treated with NO,H2O2 or IBA alone. The IBA-mediated effect was differ... |
| Keywords: | Tagetes erecta L |
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