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| Mini-Tn10转座子构建欧文氏杆菌突变体的研究 | |
| 引用本文： | 石岩,刘正初,徐君飞,张居作,李炫.Mini-Tn10转座子构建欧文氏杆菌突变体的研究[J].湖北农业科学,2010,49(1). |
| 作者姓名： | 石岩 刘正初 徐君飞 张居作 李炫 |
| 作者单位： | 1. 中国农业科学院麻类研究所,长沙,410205 2. 湖南农业大学,长沙,410128 3. 广西大学,南宁,530005 |
| 基金项目： | 国家"863"计划项目 |
| 摘 要： | 构建细菌突变体是发现新基因、分析基因功能、了解某些生物机理的重要途径。本研究通过转座子Mini-Tn10对迄今报道草本纤维提取效率最高的菌株胡萝卜软腐欧文氏杆菌(Erwinia carotovor)变异菌株CXJZU120进行随机突变,获得5 523个突变体。采用非纤维素降解实效法和水解圈法等功能性鉴定,筛选出3个非纤维素降解活性降低或丧失的突变体,为深入研究非纤维素降解机理,寻找与非纤维素降解相关基因并构建新一代高效菌株奠定了基础。 |
| 关 键 词： | 欧文氏杆菌 转化 转座子 突变体 |
Construction of Mutant of Erwinia carotovor by Transposon Mini-Tn10 Insertion |
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| SHI Yan,LIU Zheng-chu,XU Jun-fei,ZHANG Ju-zuo,LI Xuan.Construction of Mutant of Erwinia carotovor by Transposon Mini-Tn10 Insertion[J].Hubei Agricultural Sciences,2010,49(1). | |
| Authors: | SHI Yan LIU Zheng-chu XU Jun-fei ZHANG Ju-zuo LI Xuan |
| Abstract: | Construction of bacterial mutant is an important way to discover new genes,analyze functions of genes and find out certain biological mechanism.In this research,we obtained 5 523 mutants by Mini-Tn10 mediated insertional mutagenesis in CXJZU 120,the mutant of Erwinia carotovor which was the most powerful strain reposed for the bio-extraction of herbaceous materials.Going with halo-producing assay we used the mutants to degrade non-cellulose and screened three non-cellulose degrading reduced or free mutants.It laid the foundation for studying the mechanism of non-cellulose degrading,searching the genes which were related to biological non-cellulose degrading and constructing a new generation of efficient strain. |
| Keywords: | Tn10 Erwinia carotovor transformation transposon Tn10 mutant |
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