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| 不同增菌液和培养基对沙门菌分离效果的比较研究 | |
| 引用本文： | 陈惠娟,张纯萍,李金贵,宁宜宝,宋立,董剑辉.不同增菌液和培养基对沙门菌分离效果的比较研究[J].中国家禽,2011,33(24). |
| 作者姓名： | 陈惠娟 张纯萍 李金贵 宁宜宝 宋立 董剑辉 |
| 作者单位： | 1. 中国兽医药品监察所,北京100081;扬州大学兽医学院,江苏扬州225009 2. 中国兽医药品监察所,北京,100081 3. 扬州大学兽医学院,江苏扬州,225009 4. 华南农业大学兽医学院,广东广州,510642 |
| 基金项目： | 北京市科技新星计划(2009B52) |
| 摘 要： | 通过对各种增菌液和培养基的比较以及组合筛选,选择一种较为有效和简便的分离沙门菌的方法.采用6种常用的选择性培养基对9种血清型的沙门菌以及常见的共生肠道菌大肠杆菌和阴沟肠杆菌分别进行培养,根据生长特点对选择性培养基进行初步筛选,然后对3种常用选择性增菌液进行筛选,将筛选出的增菌液与培养基进行不同组合,检测各血清型沙门菌与大肠杆菌、阴沟肠杆菌的混合物在不同组合中的增菌和分离效果,并对其进行评价.研究结果表明,最佳分离培养方案为样品经SC和TTB增菌后再用显色培养基或者XLT4培养基进行选择性培养.本研究结果优化了沙门菌的常规分离培养方法,可用于不同样品中沙门菌的分离培养,提高沙门菌分离率. |
| 关 键 词： | 沙门菌 选择性增菌液 选择性培养基 分离效果 |
Effect of Different Enrichment Broth and Culture Medium on Isolation Effects of Salmonella |
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| CHEN Huijuan , ZHANG Chunping , LI Jingui , NING Yibao , SONG Li , DONG Jianhui.Effect of Different Enrichment Broth and Culture Medium on Isolation Effects of Salmonella[J].China Poultry,2011,33(24). | |
| Authors: | CHEN Huijuan ZHANG Chunping LI Jingui NING Yibao SONG Li DONG Jianhui |
| Institution: | CHEN Huijuan1,2,ZHANG Chunping1,LI Jingui2,NING Yibao1,SONG Li1,DONG Jianhui3(1. China Institute of Veterinary Drug Control,Beijing 100081,2.College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,3.College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong 510642) |
| Abstract: | To select a general method through the comparison of various enrichment broth and culture medium and combinations of the two respectively,which can isolate Salmonella effectively and conveniently,Salmonella of nine serotypes and two common symbiosis enterobacteria(Escherichia coli and Enterobacter cloacae) were cultured and isolated with six commonly selective culture mediums respectively. Selective culture mediums were initially selected according to the growth condition. Three selective enrichment broth w... |
| Keywords: | Salmonella selective enrichment broth selective culture medium isolation effect |
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