| 1个小麦NBS类抗病基因同源cDNA序列的克隆与鉴定 |
| |
| 引用本文： | 王海燕,刘大群,杨文香,李在峰,张立荣,张娜.1个小麦NBS类抗病基因同源cDNA序列的克隆与鉴定[J].植物病理学报,2009,39(5):507-513. |
| |
| 作者姓名： | 王海燕 刘大群 杨文香 李在峰 张立荣 张娜 |
| |
| 作者单位： | 河北省农作物病虫害生物防治工程技术研究中心, 河北农业大学植物保护学院, 保定 071001 |
| |
| 基金项目： | 河北省自然科学基金，河北省教育厅，国家自然科学基金 |
| |
| 摘 要： | 利用cDNA末端快速扩增技术对小麦抗叶锈病近等基因系TcLr35中所获得的抗病基因同源片段S2A2 5'端和3'末端序列进行扩增,并根据拼接序列设计特异性引物,进行全长基因的扩增,获得了1个通读的NBS类抗病同源基因S2A2 cDNA序列,该序列全长为3476 bp,编码866个氨基酸序列。经BLASTp比较,该片段含有NB-ARC保守结构域和多个LRR结构域,与已知植物抗病基因I2C-1、L6、RPS2等相应区域相一致。半定量RT-PCR分析表明,该基因在小麦叶片中为低丰度组成型表达。本研究在TcLr35小麦中成功获得了抗病同源基因,这为最终克隆小麦抗叶锈病基因Lr35奠定了基础。
|
| 关 键 词： | 小麦抗叶锈病基因 cDNA末端快速扩增技术 同源序列 |
| 收稿时间： | 2009-02-16 |
Identification and cloning of a NBS resistance gene homology cDNA sequence from wheat |
| |
| WANG Hai-yan,LIU Da-qun,YANG Wen-xiang,LI Zai-feng,ZHANG Li-rong,ZHANG Na.Identification and cloning of a NBS resistance gene homology cDNA sequence from wheat[J].Acta Phytopathologica Sinica,2009,39(5):507-513. |
| |
| Authors: | WANG Hai-yan LIU Da-qun YANG Wen-xiang LI Zai-feng ZHANG Li-rong ZHANG Na |
| |
| Institution: | Biological Control Center of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China |
| |
| Abstract: | The 5' and 3' end of the resistance gene homology fragment S2A2 from TcLr35 were obtained by the rapid amplification cDNA ends (RACE). The gene specific primers were designed based on the spliced sequence, and the full length sequence of S2A2 obtained was 3476bp encoding 866 amino acids. BLASTp a-nalysis, the deduced amino acids of S2A2 protein consisted of a NB-ARC conserved domain and a leucine-rich repeats (LRR) domain, which were identical to the conserved domains of plant resistance genes such as 12C-1, L6, and RPS2. The S2A2 gene appeared not to be induced by Puccinia triticina and was a constitutive gene with low abundance in the wheat leaf tissue by semi-quantitative RT-PCR. In this study, the resistance homology sequence were obtained in TcLr35, which provide the shortcut for cloning of wheat leaf rust resistance gene Lr35. |
| |
| Keywords: | wheat leaf rust resistance gene rapid amplification cDNA ends (RACE) homology sequence |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
