| 盐胁迫下陆地棉耐盐品种根系的抑制消减文库构建 |
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| 引用本文： | 叶武威,赵云雷,王俊娟,樊保香.盐胁迫下陆地棉耐盐品种根系的抑制消减文库构建[J].棉花学报,2009,21(5):339-345. |
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| 作者姓名： | 叶武威 赵云雷 王俊娟 樊保香 |
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| 作者单位： | 中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,河南,安阳,455000 |
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| 基金项目： | 中央级公益性科研院所基本科研业务专项 |
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| 摘 要： | 采用抑制消减(SSH)杂交技术构建了陆地棉耐盐品种中棉所35在盐(0.4%NaCl)胁迫后1 d、3 d、5 d和7 d的混合SSH文库。文库质量检测表明，差减杂交效率较高，质量较好。随机挑选200个阳性克隆进行测序，获得160余条高质量的表达序列标签（EST）。对序列进行BLAST比对及功能注释，其中有62条unigene 未获得同源性匹配, 42条与未知功能的序列同源性较高；其余56条功能已知的unigene中，与抗逆直接相关的基因有14个,占8.8%，参与新陈代谢及信号转导的基因有11条和10条, 分别占6.9%和6.3%，参与转录调控的基因8条，占5.0%。最后，对这些基因在盐胁迫中的作用进行了注释与讨论。
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| 关 键 词： | 棉花 抗逆性 耐盐基因 SSH文库 |
| 收稿时间： | 2008-10-24; |
Construction of SSH Library on Root System of Salinity-Tolerance Variety(G. hirsutum L.) under the Stress of Salinity |
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| YE Wu-wei,ZHAO Yun-lei,WANG Jun-juan,FAN Bao-xiang.Construction of SSH Library on Root System of Salinity-Tolerance Variety(G. hirsutum L.) under the Stress of Salinity[J].Cotton Science,2009,21(5):339-345. |
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| Authors: | YE Wu-wei ZHAO Yun-lei WANG Jun-juan FAN Bao-xiang |
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| Institution: | Cotton Research Institute of the Chinese Academy of Agriculture Sciences;Key Laboratory of Cotton Genetic Improvement;Ministry of Agriculture;Anyang;Henan 455000;China |
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| Abstract: | A SSH library from the salt tolerant upland cotton cultivar,CCRI 35,was constructed using suppression subtractive hybridization(SSH) and pGEM-T Easy Vector under salt(0.4% of NaCl) stress at the first,third,fifth and seventh day after the beginning of salination.The library was evaluated to be of good quality with high efficiency of SSH.Total 200 positive clones were randomly selected and then sequenced,and 160 high quality sequences were obtained.According to BLAST screening and functional annotation,there... |
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| Keywords: | cotton stress tolerance salt tolerance gene SSH library |
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