| 一株信鸽新城疫强毒的分离鉴定和分子特性分析 |
| |
| 引用本文： | 袁小远,王友令,徐怀英,欧阳文军,贾强,何叶峰,秦卓明.一株信鸽新城疫强毒的分离鉴定和分子特性分析[J].农业生物技术学报,2007,15(6):942-946. |
| |
| 作者姓名： | 袁小远 王友令 徐怀英 欧阳文军 贾强 何叶峰 秦卓明 |
| |
| 作者单位： | 山东省农业科学院家禽研究所,济南,250100 |
| |
| 基金项目： | 山东省科技攻关项目;山东省自然科学基金 |
| |
| 摘 要： | 从发病鸽的体内分离到一株新城疫病毒（Newcastle Disease Virus，NDV）PB9601，经病毒蚀斑克隆纯化后动物回归试验可产生典型的NDV病变，其MDT、ICPI、IVPI分别为50.5、1.65和2.36，表明为NDV强毒。对其F和HN蛋白分别进行基因克隆测序，发现F蛋白多肽裂解位点的序列为111 KRQKRF 117，符合鸽源NDV强毒氨基酸基序。F基因分型及同源性比较显示：PB9601与YN-03、TW-98等鸽源毒株属于VI型，其F基因与基因Ⅱ型疫苗株LaSota氨基酸同源性为88.6%，与基因Ⅰ型疫苗株V4的氨基酸同源性为91.0%，与基因Ⅸ型传统强毒F48E9等的氨基酸同源性为90.6%；与国内鸽分离株GX-05、YN-03、TW-98的氨基酸同源性分别为92.1%、95.5、98.6%、与国外鸽分离株Capital3-97、Tigre6-99、1166-00、IT-227-82、2736-00的同源性分别为93.3%、91.9%、94.6%、95.1%、93.6%。HN基因氨基酸同源比较显示：PB9601与LaSota、F48E9、V4等的氨基酸同源性分别为87.2%、89.2%、87.6%；与国外鸽分离株1166-00、IT-227-82、2736-00的同源性为93.7%、95.5%、92.3%。由此看出PB9601与国内外分离株、LaSota、F48E9、V4等相比，具有明显的地域性和种属性。
|
| 关 键 词： | 鸽 新城疫病毒 F基因 HN基因 分子特性 |
| 文章编号： | 1006-1304（2007）06-0942-05 |
| 收稿时间： | 2007-03-09 |
| 修稿时间： | 2007-04-12 |
Molecular Characterization of One Newcastle disease virus Isolate Collected from Homing Pigeon |
| |
| YUAN Xiao-yuan,WANG You-ling,XU Huai-ying,OUYANG Wen-jun,JIA Qiang,HE Ye-feng,QIN Zhuo-ming.Molecular Characterization of One Newcastle disease virus Isolate Collected from Homing Pigeon[J].Journal of Agricultural Biotechnology,2007,15(6):942-946. |
| |
| Authors: | YUAN Xiao-yuan WANG You-ling XU Huai-ying OUYANG Wen-jun JIA Qiang HE Ye-feng QIN Zhuo-ming |
| |
| Abstract: | A Newcastle disease virus field strain PB9601 was isolated from a pigeon in Beijing which can form typical pathological changes through regression experiment after plaque-purification. NDV PB9601 was determined as a virulent strain with MDT of 50.5h , ICPI of 1.65, IVPI of 2.36 respectively. The F and HN gene were cloned and sequenced. The sequence analysis of F gene indicated that the amino acid sequence is 111 KRQKRF 117 in the F protein cleavage site in PB9601 strain which is identical to virulent strains of NDV. The homology analysis of F and HN gene sequences compared to reference isolates from GenBank indicated that: PB9601 belongs to genotype VI with YN-03 and TW-98；The F protein amino acid sequence has homologies of 88.6%,91.0%,90.6% with LaSota,V4,F48E9 and of 92.1%, 95.5%, 98.6% with isolates GX-05,YN-03,TW-98 in China and of93.3%、91.9%、94.6%、95.1%、93.6% with isolates Capital3-97、Tigre6-99、1166-00、IT-227-82、2736-00 abroad. When HN genes were compared, PB9601 demonstrated higher homologies of 91.9%, 93.3%, 94.6%, 95.1%, 93.6% with isolated strains Tigre6-99, Capital3-97, 1166-00, IT-227-82, 2736-00 abroad , but lower homologies of 87.2% ,89.2%, 87.6% with LaSota , F48E9 and V4.The above results show PB9601 has obvious regional and genus traits compared with isolated strains LaSota ,F48E9 and V4 . |
| |
| Keywords: | pigeon Newcastle disease virus F gene HN gene molecular characterization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《农业生物技术学报》浏览原始摘要信息 |
|
