| t10,c12-CLA对猪皮下脂肪和背最长肌组织脂类代谢的影响 |
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| 引用本文： | 杜瑞平,高民,卢德勋.t10,c12-CLA对猪皮下脂肪和背最长肌组织脂类代谢的影响[J].饲料工业,2010,31(19). |
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| 作者姓名： | 杜瑞平 高民 卢德勋 |
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| 作者单位： | 1. 内蒙古农业大学动物科学学院,010018,内蒙古,呼和浩特
2. 内蒙古农牧业科学院动物营养所 |
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| 基金项目： | 国家"973"重点基础研究发展计划 |
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| 摘 要： | 试验首先建立了30日龄猪皮下脂肪和背最长肌组织块体外培养体系,共设2个处理,处理1为阴性对照(添加0.1mmol/l牛血清白蛋白,BSA),处理2添加100μmol/l的t10,c12-CLA,每个处理设6个平行,接种时即加入BSA与t10,c12-CLA进行处理至第10d,试验结束后收集细胞保存备用。采用荧光定量PCR技术及试剂盒检测研究t10,c12-CLA对猪皮下和背最长肌脂肪代谢关键酶(FAS、ME、LPL、HSL)和激素(INSR、GHR)及甘油三酯(TG)合成的影响。结果表明:(1)100μmol/lt10,c12-CLA显著降低了猪皮下脂肪TG含量并提高了猪背最长肌的TG含量(P0.05);(2)100μmol/lt10,c12-CLA显著抑制了猪皮下脂肪FAS、INSR与背最长肌HSL和LPL的基因表达,促进了猪皮下脂肪HSL和背最长肌INSR的基因表达(P0.05);(3)本研究结果从脂肪代谢关键酶类与调控激素方面揭示了t10,c12-CLA对猪皮下和背最长肌组织脂肪代谢与沉积的差异性调控机制,进一步证实了t10,c12-CLA抑制猪皮下脂肪沉积同时提高肌内脂肪含量。
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| 关 键 词： | t10 c12-CLA 背最长肌 皮下脂肪 脂肪代谢关键酶 调控激素 猪 |
Studies on effects of t10,c12-CLA on lipid metabolism of subcutaneous and longissimus muscle tissues in pig |
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| Du Ruiping,Gao Min,Lu Dexun.Studies on effects of t10,c12-CLA on lipid metabolism of subcutaneous and longissimus muscle tissues in pig[J].Feed Industry,2010,31(19). |
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| Authors: | Du Ruiping Gao Min Lu Dexun |
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| Institution: | Du Ruiping,Gao Min,Lu Dexun |
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| Abstract: | In vitro tissue culture system of subcutaneous(SC) adipose and longissimus muscle taken from 30-days pig were constructed firstly,0.1 mmol/l bovine serum albumin (BSA,as control) ,100μmol/L t10,c12-CLA were added to the cultures for 10 d,six replicates were performed for each treatment. At the end of a 10-d treatment period,cells were harvested and stored at-70℃. Real-time RT-PCR and kit assay were used to characterize the effects of t10,c12-CLA on the lipogenic and lipolytic enzymes ,regulation hormones,TG content in longissimus muscle and SC adipose of pig. The results showed that:the mRNA abundance of FAS and INSR,TG content in SC adipose tissue and the mRNA abundance of HSL and LPL in longissimus muscle tissue were decreased by adding t10,c12-CLA (P<0.05);by contrast,HSL mRNA abundance in SC adipose tissue and the mRNA abundance of INSR,TG content in longissimus muscle tissue were increased by adding t10,c12-CLA(P<0.05). These results illustrated that the effects of t10,c12-CLA on lipid metabolism and deposition of SC adipose tissue differed from that on longissimus muscle tissue ,and confirmed t10,c12-CLA inhibited the lipid deposition of SC adipose and promoted the lipid deposition of longissimus muscle in pig further. |
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| Keywords: | t10 c12-CLA |
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