| 广州市售肉类食品源大肠埃希菌耐药性分析 |
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| 引用本文： | 曾莉,黄仕凤,刘思玲,王晶,刘健华.广州市售肉类食品源大肠埃希菌耐药性分析[J].华南农业大学学报,2017,38(2):27-31. |
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| 作者姓名： | 曾莉 黄仕凤 刘思玲 王晶 刘健华 |
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| 作者单位： | 1. 华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室,广东广州,510642;2. 佛山市正典生物技术有限公司,广东佛山,528138 |
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| 基金项目： | 973计划(2013CB127200) |
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| 摘 要： | 【目的】了解广州市售肉类食品源大肠埃希菌的污染和耐药情况。【方法】自2011年7月至8月,从广州市各大农贸市场和超市采集市售肉类样品310份,其中猪肉253份,鸡肉57份。采用选择性培养基分离鉴定大肠埃希菌Escherichia coli,琼脂稀释法测定大肠埃希菌对19种抗菌药物的最小抑菌浓度(MIC)。【结果】310份样品中共分离到213株大肠埃希菌,分离率为68.7%,其中猪肉源177株,鸡肉源36株。受试菌株对复方新诺明、链霉素和四环素的耐药率超过75.0%,对氨苄西林、萘啶酸、氯霉素、新霉素、氟苯尼考、磷霉素、环丙沙星、安普霉素、恩诺沙星、庆大霉素和头孢唑啉的耐药率为10.0%~60.0%,而对头孢西丁、头孢曲松、头孢他啶、黏菌素和阿米卡星表现较为敏感,耐药率小于5.0%。鸡肉源大肠埃希菌对头孢唑啉、头孢西丁、头孢曲松、头孢他啶和磷霉素的耐药率均高于猪肉源大肠埃希菌,差异极显著(P0.01)。213株大肠埃希菌中82.2%为多重耐药菌。【结论】广州市售肉类食品存在较为严重的多重耐药大肠埃希菌污染,且鸡肉源大肠埃希菌耐药性较猪肉源大肠埃希菌严重。
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| 关 键 词： | 大肠埃希菌 肉类食品源 琼脂稀释法 耐药性 |
| 收稿时间： | 2016/5/4 0:00:00 |
Antimicrobial activity of Escherichia coli from retail meat in Guangzhou |
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| ZENG Li,HUANG Shifeng,LIU Siling,WANG Jing,LIU Jianhua.Antimicrobial activity of Escherichia coli from retail meat in Guangzhou[J].Journal of South China Agricultural University,2017,38(2):27-31. |
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| Authors: | ZENG Li HUANG Shifeng LIU Siling WANG Jing LIU Jianhua |
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| Abstract: | Objective] To understand the contamination status and antimicrobial activity of Escherichia coli in retail meat sold in Guangzhou .Method]We collected 310 meat samples including 253 retail pork and 57 chicken meat from markets and supermarkets in Guangzhou from July to August , 2011.E.coli strains were isolated and identified using the selective media .The susceptibilities of E.coli strains to 19 common antibiotics represented by the minimum inhibitory concentrations ( MIC ) were tested using the agar dilution method .Result]Totally 213 E.coli strains were isolated from 310 meat samples with the detection frequency of 68.7%, including 117 pork isolates and 36 chicken meat isolates .More than 75.0%isolates were resistant to sulfamethoxazole/trimethoprim , streptomycin and tetracycline , 10.0%to 60.0%isolates were resistant to ampicillin , nalidixic acid , chloramphenicol , neomycin , florfenicol , fosfomycin, ciprofloxacin, apramycin, enrofloxacin, gentamicin and cefazolin, and the isolates were sen-sitive to cefoxitin , ceftriaxone , ceftazidime , colistin and amikacin with below 5.0% being resistant strains.Isolates from chicken meat showed significantly higher resistance to cefazolin , cefoxitin, ceftriax-one, ceftazidime and fosmycin compared to pork isolates ( P <0.01 ) .There were 82.2% multi-drug resistant strains of 213 E.coli strains.Conclusion]Retail meat sold in Guangzhou were severely contami-nated by multi-drug resistant E.coli, and chicken meat isolates showed higher resistance compared to pork isolates . |
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| Keywords: | Escherichia coli retail meat origin agar dilution method antimicrobial activity |
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