| 猪心脏脂肪酸结合蛋白基因的克隆和测序分析 |
| |
| 引用本文： | 陈小波,廖国周,花群义,葛长荣.猪心脏脂肪酸结合蛋白基因的克隆和测序分析[J].云南农业大学学报,2004,19(4):378-380. |
| |
| 作者姓名： | 陈小波 廖国周 花群义 葛长荣 |
| |
| 作者单位： | [1]云南农业大学动物科学技术学院，云南昆明650201 [2]云南出入境检验检疫局技术中心，云南昆明650228 |
| |
| 基金项目： | 云南省十五攻关项目(2001NG40) |
| |
| 摘 要： | 根据GeneBank发表的猪心脏脂肪酸结合蛋白（heart fatty acid-binding protein,H-FABP）基因序列,设计合成一对引物,从杜长大三元杂交猪的腰大肌中提取总RNA,对H-FABP基因进行RT-PCR扩增,产物经琼脂糖电泳分析,呈现一条约124 bp的条带,回收纯化后,将其克隆至pMD18-T质粒载体中,然后进行核苷酸序列分析。与GeneBank中报道的H-FABP基因比较后发现,该基因片段与其它的核苷酸的同源性为94%～100%,推导的氨基酸的同源性为100%.
|
| 关 键 词： | 猪 心脏脂肪酸结合蛋白基因 RT-PCR 基因克隆 序列分析 |
| 文章编号： | 1004-390X(2004)04-0378-03 |
| 收稿时间： | 2004-6-15 |
Cloning and Sequencing of Heart Fatty Acid-binding Protein Gene in Porcine |
| |
| CHEN Xiao-bo,LIAO Guo-zhou,HUA Qun-yi,GE Chang-rong.Cloning and Sequencing of Heart Fatty Acid-binding Protein Gene in Porcine[J].Journal of Yunnan Agricultural University,2004,19(4):378-380. |
| |
| Authors: | CHEN Xiao-bo LIAO Guo-zhou HUA Qun-yi GE Chang-rong |
| |
| Institution: | CHEN Xiao-bo~1,LIAO Guo-zhou~1,HUA Qun-yi~2,GE Chang-rong~1 |
| |
| Abstract: | Heart fatty acid-binding protein gene isolated from porcine (Duroc×Landrace×Yorkshire) muscle tissue, were amplified by RT-PCR. The product of 0.124 kb fragment in length was obtained as expected. Then, the amplified fragment was cloned into the pDM18-T vector and identified by PCR and sequence. The results demonstrated that nucleotide homology with other published H-FABP genes was between 94%～100%, and deduced amino acid homology was between 100% respectively. |
| |
| Keywords: | porcine H-FABP gene RT-PCR gene cloning sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《云南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《云南农业大学学报》下载免费的PDF全文 |
