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| 2007年华北地区H3N8亚型马流感病毒的分离与鉴定 | |
| 引用本文： | 蒋桃珍,刘月焕,林健,韩春华,潘洁,赖平安,韦海涛,祝俊杰,赵景义,马志军,毕丁仁.2007年华北地区H3N8亚型马流感病毒的分离与鉴定[J].中国兽医学报,2010,30(5). |
| 作者姓名： | 蒋桃珍 刘月焕 林健 韩春华 潘洁 赖平安 韦海涛 祝俊杰 赵景义 马志军 毕丁仁 |
| 作者单位： | 1. 华中农业大学动物医学院,湖北省预防兽医学重点实验室,湖北武汉430070;中国兽医药品监察所,北京100081 2. 北京市农林科学院畜牧兽医研究所,北京,100097 3. 北京出入境检验检疫局,北京,100029 4. 北京市畜牧兽医总站,北京,100029 5. 华中农业大学动物医学院,湖北省预防兽医学重点实验室,湖北武汉430070 |
| 基金项目： | 北京市委组织部人才培养基金资助项目(20081D020-0500056) |
| 摘 要： | 2007年10月,华北地区某赛马场的马同时发生了以发烧、流水样鼻汁或脓性分泌物、咳嗽等临床症状为主的疾病,疑似马流行性感冒。采集患病赛马的鼻腔分泌物,发病期和发病后14d血清,经鸡胚接种法分离病毒,并用鸡红细胞血凝抑制试验(HI)、神经氨酸酶抑制试验(NI)、病毒回归试验、血清学检测和基因序列分析对分离的病毒进行了系统鉴定。结果表明分离的毒株(A/equine/Huabei/1/2007(H3N8)为马源H3N8亚型马流感病毒,基因型属于美洲分支。我们通过动物回归感染试验建立起分离毒株的实验感染模型。 |
| 关 键 词： | 马流感病毒 H3N8 马 分离鉴定 |
Isolation and Identification of Equine Influenza Virus Subtype H3N8 causing an outbreak in Northern China in 2007 |
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| JIANG Tao-zhen,LIU Yue-huan,LIN Jian,HAN Chun-hua,PAN Jie,LAI Ping-an,WEI Hai-tao,ZHU Jun-jie,ZHAO Jing-yi,MA Zhi-jun,BI Ding-ren.Isolation and Identification of Equine Influenza Virus Subtype H3N8 causing an outbreak in Northern China in 2007[J].Chinese Journal of Veterinary Science,2010,30(5). | |
| Authors: | JIANG Tao-zhen LIU Yue-huan LIN Jian HAN Chun-hua PAN Jie LAI Ping-an WEI Hai-tao ZHU Jun-jie ZHAO Jing-yi MA Zhi-jun BI Ding-ren |
| Institution: | JIANG Tao-zhen1,5,LIU Yue-huan2,LIN Jian2,HAN Chun-hua2,PAN Jie2,LAI Ping-an3,WEI Hai-tao4,ZHU Jun-jie4,ZHAO Jing-yi4,MA Zhi-jun4,BI Ding-ren1 (1.College of Veterinary Medicine,Huazhong Agricultural University,Key Laboratory of Preventive Medicine of Hubei Province,Wuhan 430070,China,2.Institute of Animal Husb,ry , Veterinary Medicine,Beijing Academy of Agriculture , Forestry Sciences,Beijing 100097,3.Beijing Entry-Exit Inspection , Quarantine Bureau,Beijing 100029,4.Beijing Municipal Hu... |
| Abstract: | In October 2007,an outbreak of severe respiratory disease with the developing clinical symptoms such as depression,anorexia,dry cough,high fever and mucopurulent nasal discharge occurred in race horses in Northern China.We took the samples of nasal discharge and paired sera and examined the presence of equine influenza virus and autibody.Embryonated SPF eggs were used for virus inoculation from nasopharyngeal swabs,viral growth was monitored by haemagglutination assay (HA) and the isolate was characterized ... |
| Keywords: | Equine influenza H3N8 horses isolation and identification |
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