| 高效玉米秸秆降解菌复合系的构建 |
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| 引用本文： | 郭夏丽,程小平,杨小丽,王岩.高效玉米秸秆降解菌复合系的构建[J].中国农学通报,2010,26(7):261-266. |
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| 作者姓名： | 郭夏丽 程小平 杨小丽 王岩 |
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| 作者单位： | 1. 郑州大学化工与能源学院,郑州,450001
2. 湖北职业技术学院护理系,湖北孝感,432000 |
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| 基金项目： | 河南省科技攻关计划项目 |
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| 摘 要： | 依据秸秆降解所需的酶系及微生物之间的协调性,通过增加菌种多样性,实现秸秆降解菌剂的高效稳定;首先采用驯化和分离纯化的方法,得到不同的天然菌群和单菌株,然后将其进行有效的组配和限制性继代培养,以构建秸秆降解复合菌剂。结果表明,通过单菌种组配,得到由纤维素降解菌Y11-4和X2、木质素降解菌L3、酵母菌J6组成的秸秆降解率最高的菌种组合:Y11-4+X2+L3+J6。以秸秆为唯一碳源进行限制性继代培养,依据pH恒定,得到5种分别来自腐烂秸秆、森林土、菜园土、竹林土及麦田土的驯化系。通过菌系组配,得到了一个来源于森林土、竹林土和腐烂秸秆的驯化系组合,其秸秆降解率为38.3%。进一步将驯化系组合与单菌株组合复配并限制性继代培养,得到的秸秆降解复合菌剂在14天内秸秆降解率达到45.7%。结果表明,通过增加菌种多样性和继代培养构建秸秆降解复合菌剂是行之有效的方法。
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| 关 键 词： | 玉米秸秆降解 复合菌系 驯化系 |
| 收稿时间： | 2009-12-02 |
| 修稿时间： | 2009-12-17 |
Construction of Composite Consortia with High Capacity of Lignocellulose Degradation |
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| Guo Xiali,Cheng Xiaoping,Yang Xiaoli,Wang Yan.Construction of Composite Consortia with High Capacity of Lignocellulose Degradation[J].Chinese Agricultural Science Bulletin,2010,26(7):261-266. |
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| Authors: | Guo Xiali Cheng Xiaoping Yang Xiaoli Wang Yan |
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| Institution: | 1Department of Environmental Science, College of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001; 2Department of Nursing, Hubei Vocational Technical College, Xiaogan Hubei 432000) |
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| Abstract: | Based on the different enzyme complexes required for straw degradation and the coordination between the microbial populations, a highly efficient and stable straw degrading composite microbial system should be obtained by increasing the strain diversity. Firstly the different natural flora and single strain were screened by use of domestication and purification methods, and then the complex microbial system was developed through mixing and the restrictive subculture; The results showed that a mixture consisting of cellulose-decomposing fungi Y11-4 and X2, lignin-degrading fungus L3 and yeast J6 was obtained according to the stalk-degrading efficiency. With maize stalk as sole carbon source, the five kinds of domesticated strains from forest soil, cornfield soil, bamboo soil, rotten cornstalk and garden soil were found by the techniques of subculture and using pH stability as a criterion. An efficient straw-degrading composite microbial system was constructed by mixing domesticated strains from forest soil, bamboo soil and rotten corn stalk with Y11-4＋X2＋ L3＋J6 and continuous limited subculture. And its straw degradation efficiency was up to 45.7% in 14 days. The built of straw degrading composite microbial system through increasing the strain diversity and subculture was feasible. |
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| Keywords: | maize stalk degradation composite microbial system domesticated strains |
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