| 玉米弯孢叶斑病菌Fus3/Kss1-MAPK级联途径的相关基因鉴定与结构特征分析 |
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| 引用本文： | 赵丰舟,刘震,侯巨梅,崔佳,左豫虎,刘铜.玉米弯孢叶斑病菌Fus3/Kss1-MAPK级联途径的相关基因鉴定与结构特征分析[J].农学学报,2017,7(1):84-90. |
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| 作者姓名： | 赵丰舟 刘震 侯巨梅 崔佳 左豫虎 刘铜 |
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| 作者单位： | 黑龙江八一农垦大学农学院植物病理与应用微生物研究所,黑龙江大庆,163319 |
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| 基金项目： | 黑龙江八一农垦大学研究生创新科研项目(YJSCX2015-Y06)、国家自然科学基金资助项目(31272026) |
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| 摘 要： | 植物病原真菌Fus3/Kss1-MAPK级联途径在调控病原菌附着胞形成和致病性等方面起重要作用。为了明确Fus3/Kss1-MAPK级联途径在玉米弯孢叶斑病菌中的调控作用,笔者采用生物信息学方法,对玉米弯孢叶斑病菌全基因组中的Fus3/Kss1-MAPK级联途径的相关基因进行鉴定并对其分子结构特征分析。结果表明:从玉米弯孢叶斑病菌中鉴定出Fus3/Kss1-MAPK级联途径中3个蛋白激酶基因Clf、Map2k、Clk1和一个锚定蛋白基因Cl Ste50。蛋白序列分析以及进化树构建等表明它们分别与来源其他植物病原真菌的Fus3/Kss1-MAPK途径中的激酶蛋白和锚定蛋白具有相似的结构特征和保守结构域,有较高同源性和较近的亲缘进化关系。玉米弯孢叶斑病菌Fus3/Kss1-MAPK途径的相关蛋白激酶Clk1、Map2k、Clf和锚定蛋白Clste50与其他真菌Fus3/Kss1-MAPK途径的相关蛋白结构相似,推断有相似的功能。
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| 关 键 词： | 弯孢叶斑病菌 Fus3/Kss1-MAPK 级联途径 结构特征 |
| 收稿时间： | 2016/3/16 0:00:00 |
| 修稿时间： | 2016/6/26 0:00:00 |
Identification and Structure Characteristics of Related Genes of Fus3/Kss1-MAPK Pathway from Curvularia lunata |
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| Zhao Fengzhou,Liu Zhen,Hou Jumei,Cui Jia,Zuo Yuhu,Liu Tong.Identification and Structure Characteristics of Related Genes of Fus3/Kss1-MAPK Pathway from Curvularia lunata[J].Journal of Agriculture,2017,7(1):84-90. |
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| Authors: | Zhao Fengzhou Liu Zhen Hou Jumei Cui Jia Zuo Yuhu Liu Tong |
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| Abstract: | The cascade pathway of Fus3/Kss1-MAPK in plant pathogenic fungi plays an important role in regulating appressorium development and pathogenicity.In order to investigate the role of Fus3/Kss 1-MAPK cascade pathway in'Curvularia lunata',we used bioinformatics methods to identify the related gene of the Fus3/Kss1-MAPK cascade pathway from'Curvularia lunata'in genome-wide and analyzed the molecular structure characteristics of Fus3/Kss 1-MAPK related gene.The result showed that three protein kinases genes of Clf,Map2K,Clk1 and an ankyrin gene Clste50 were identified based on genome of'Curvularia lunata'.The protein sequence analysis and phylogenetic tree construction showed that Clf,Map2k,Clk1 and Clste50 in 'Curvularia lunata'all contained similar structural features and conservative domains with those of other plant pathogenic fungi,and had high homology and close phylogenetic relationships.The related protein kinases genes Clf,Map2K,Clk1 and an ankyrin gene Clste50 of the Fus3/Kss1-MAPK cascade pathway from 'Curvularia lunata'were similar to the protein structures with other fungi.Those results may indicate that they have similar function in appressorium development and pathogenicity. |
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| Keywords: | Curvularia lunata Fus3/Kss1-MAPK Cascade Pathway Structure Characteristics |
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