| 高血压心力衰竭大鼠动物模型的研制 |
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| 作者姓名： | 李欣春 梁昊 胡志希 胡思远 文阳 钟森杰 李琳 |
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| 作者单位： | 湖南中医药大学中医诊断研究所, 湖南 长沙 410007,湖南中医药大学中医诊断研究所, 湖南 长沙 410007,湖南中医药大学中医诊断研究所, 湖南 长沙 410007,湖南中医药大学中医诊断研究所, 湖南 长沙 410007,湖南中医药大学中医诊断研究所, 湖南 长沙 410007,湖南中医药大学中医诊断研究所, 湖南 长沙 410007,湖南中医药大学中医诊断研究所, 湖南 长沙 410007 |
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| 基金项目： | 国家自然科学基金项目（81373550）。 |
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| 摘 要： | 目的 研制高血压引起的心衰大鼠动物模型。方法 将18只Dahl盐敏感性大鼠分为正常组和模型组并分别使用0.3% NaCl低盐饲料和8% NaCl高盐饲料喂养大鼠20周，观察大鼠行为及体征，检测大鼠血压、血清氨基末端脑钠肽前体（N-terminal Pro-brain natriuretic peptide，NT-proBNP）含量，彩色超声多普勒检测大鼠左室射血分数（left ventricular ejection fraction，LVEF）和左室短轴缩短率（left ventricular fractional shortening，LVFS）及电镜观察HE染色心肌细胞以及肾脏组织等。结果 正常组大鼠血压一直维持在140 mmHg左右，而模型组大鼠从进食高盐饲料后血压保持持续上升，最高可达250 mmHg；与正常组比较，模型组的NT-proBNP、LVEF、LVFS在统计学上有显著差异（P<0.01）。结论 本研究制作的动物模型可较好地模拟正常大鼠继发高血压病进而发展成高血压心衰大鼠模型的过程，可用于高血压心力衰竭疾病的研究。
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| 关 键 词： | 高血压 心力衰竭 Dahl盐敏感性大鼠 大鼠动物模型 |
| 收稿时间： | 2016/9/13 0:00:00 |
Building of Hypertensive Heart Failure Rat Models |
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| Authors: | LI Xinchun LIANG Hao HU Zhixi HU Siyuan WEN Yang ZHONG Senjie and LI Lin |
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| Institution: | Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China,Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China and Diagnostic Institute of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China |
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| Abstract: | Objective To build the rat model of hypertension heart failure. Methods Eighteen Dahl salt sensitive rats were divided into normal group and model group feed by 0.3% NaCl low-salt and 8% NaCl high-salt for 20 weeks, respectively. After 20 weeks, the daily behavior and physical signs of the rats were observed. The blood pressure, serum N-terminal-pro-brain-natriuretic-peptide (NT-proBNP) content of the rats were observed. The left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) were measured by color doppler ultrasound instrument. The HE dyed myocardial cells and kidney tissue were observed by electron microscope of the rats. Results Blood pressure of normal group rats maintained at about 140 mmHg, and blood pressure of the model group rats kept rising up to 250 mmHg after eating high-salt feed. Compared with normal group, there are significant differences in the NT-proBNP, LVEF and LVFS (P<0.01). Conclusion The rats model can well simulate the disease process that normal secondary hypertension rats develop hypertensive heart failure rats. The rat model can be used for the research of hypertensive heart failure disease. |
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| Keywords: | hypertension heart failure Dahl salt sensitive rats rat models |
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