| 鸡传染性法氏囊病免疫复合物疫苗对1日龄低母源抗体水平雏鸡免疫效果的研究 |
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| 引用本文： | 章振华,李林,景小冬,张建伟,沈佳,史爱华,郑小兰,黄凤军,姜北宇.鸡传染性法氏囊病免疫复合物疫苗对1日龄低母源抗体水平雏鸡免疫效果的研究[J].中国畜牧兽医,2015,42(9):2493-2498. |
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| 作者姓名： | 章振华 李林 景小冬 张建伟 沈佳 史爱华 郑小兰 黄凤军 姜北宇 |
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| 作者单位： | 北京市农林科学院畜牧兽医研究所, 畜禽疫病防控技术北京市重点实验室, 北京 100097 |
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| 基金项目： | 国家自然科学基金(31402230);北京市自然科学基金(6132014);北京市农林科学院创新能力建设项目(KJCX20140413) |
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| 摘 要： | 将鸡传染性法代囊病病毒(IBDV)BX株抗原和IBDV高免血清按一定比例混合,初步制备了无菌的鸡传染性法氏囊病免疫复合物疫苗1~5(分别含有32、8、4、0.5及0.125单位IBDV中和抗体),用鸡传染性法氏囊病免疫复合物疫苗1~5及BX株活疫苗分别免疫1日龄低母源抗体水平雏鸡,免疫后9 d观察法氏囊病变,免疫后28 d采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后9 d,复合物疫苗1~3免疫组鸡法氏囊正常,复合物疫苗4、5免疫组和BX株活疫苗免疫组分别有2/10、4/10和5/10的试验鸡法氏囊出现了病变;免疫后28 d,复合物疫苗1~5免疫组和BX株活疫苗免疫组IBDV中和抗体效价分别为8.34log2、9.60log2、9.21log2、7.88log2、9.50log2和9.12log2,攻毒保护率分别为90%、100%、100%、80%、90%和80%。试验结果表明鸡传染性法氏囊病免疫复合物疫苗2、3(分别含8、4单位IBDV中和抗体)免疫1日龄低母源抗体水平的雏鸡效果最好,攻毒保护率能达到100%。
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| 关 键 词： | 鸡传染性法氏囊病 免疫复合物疫苗 法氏囊 保护率 |
| 收稿时间： | 2015-03-18 |
Study on the Immunity Effect of Immune Complex Vaccine for Infectious Bursal Disease on One-day Old Low Maternal Antibody Chicken |
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| ZHANG Zhen-hua,LI Lin,JING Xiao-dong,ZHANG Jian-wei,SHEN Jia,SHI Ai-hua,ZHENG Xiao-lan,HUANG Feng-jun,JIANG Bei-yu.Study on the Immunity Effect of Immune Complex Vaccine for Infectious Bursal Disease on One-day Old Low Maternal Antibody Chicken[J].China Animal Husbandry & Veterinary Medicine,2015,42(9):2493-2498. |
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| Authors: | ZHANG Zhen-hua LI Lin JING Xiao-dong ZHANG Jian-wei SHEN Jia SHI Ai-hua ZHENG Xiao-lan HUANG Feng-jun JIANG Bei-yu |
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| Institution: | Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Municipal Academy of Agriculture and Forestry, Beijing 100097, China |
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| Abstract: | Five kinds of infectious bursal disease (IBD) immune complex (IC) vaccines were prepared with infectious bursal disease virus (IBDV) BX strain mixed with IBDV hyperimmune serum according to a certain proportion (containing 32, 8, 4, 0.5 and 0.125 units IBDV neutralizing antibody, respectively).One-day old low maternal antibody chickens were vaccinated with IBD IC vaccines 1 to 5 and BX strain live vaccine, respectively, pathological changes of the bursa of fabricius in chickens were observed at the 9th day after immunization.On the day of 28 after immunization, blood samples were taken and the IBDV neutralizing antibody were tested, meanwhile all experimental chickens were challenged with high virulent IBDV, the protective rates of vaccines were calculated.The results showed that at the 9th day after immunization, the bursa of fabricius were normal in IC vaccine 1, 2 and 3 groups, however 2/10, 4/10 and 5/10 of pathological changes of the bursa of fabricius in IC vaccine 4, 5 groups and BX strain vaccine group, respectively.At the 28th day after immunization, the IBDV neutralizing antibodies in IC vaccine 1, 2, 3, 4, 5 groups and BX strain vaccine group were 8.34log2, 9.60log2, 9.21log2, 7.88log2, 9.50log2 and 9.12log2, while 90%, 100%, 100%, 80%, 90% and 80% protection rates were provided, respectively.The results showed that IBD IC vaccines 2 and 3 (containing 8, 4 units IBDV neutralizing antibody, respectively) had the best immunity effect on one-day old low maternal antibody chickens, protection rates were both 100%. |
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| Keywords: | infectious bursal disease immune complex vaccine bursa of fabricius protection rate |
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