| 鸡传染性支气管炎病毒广西株N、M基因的克隆与序列分析 |
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| 引用本文： | 屈素洁,施开创,粟艳琼,胡杰,莫胜兰,张步娴,李军,邹联斌.鸡传染性支气管炎病毒广西株N、M基因的克隆与序列分析[J].中国畜牧兽医,2015,42(12):3119-3125. |
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| 作者姓名： | 屈素洁 施开创 粟艳琼 胡杰 莫胜兰 张步娴 李军 邹联斌 |
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| 作者单位： | 广西动物疫病预防控制中心, 南宁 530001 |
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| 基金项目： | 广西区水产畜牧兽医局科技项目(桂渔牧科1204935) |
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| 摘 要： | 为研究鸡传染性支气管炎病毒(IBV)广西流行株的遗传变异情况,本研究从广西发病鸡中分离鉴定了1株IBV。参照GenBank中IBV的核苷酸序列设计2对引物,利用RT-PCR技术对分离毒株的N、M基因进行了克隆、序列测定,并与GenBank中发表的国内外参考毒株进行比对分析。结果显示,N基因序列全长为1 230 bp,编码409个氨基酸,M基因序列全长为678 bp,编码225个氨基酸。与参考毒株相比,分离株的N基因核苷酸序列同源性为87.2%~93.3%,推导的氨基酸序列同源性为90.0%~94.4%;M基因的核苷酸序列同源性为83.6%~91.0%,推导的氨基酸序列同源性为82.7%~92.9%。在遗传进化树中,本试验分离株Guangxi156株与BJ株和LX4株两个参考株位于同一个分支上,亲缘关系较近,而与其他参考株属于不同的分支,亲缘关系较远。结果表明,本试验分离株是一株新的IBV变异株。
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| 关 键 词： | 鸡传染性支气管炎病毒 N基因 M基因 基因克隆 序列分析 |
| 收稿时间： | 2015-07-01 |
Cloning and Sequence Analysis of N and M Genes of Avian Infectious Bronchitis Virus Guangxi Strain |
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| QU Su-jie,SHI Kai-chuang,SU Yan-qiong,HU Jie,MO Sheng-lan,ZHANG Bu-xian,LI Jun,ZOU Lian-bin.Cloning and Sequence Analysis of N and M Genes of Avian Infectious Bronchitis Virus Guangxi Strain[J].China Animal Husbandry & Veterinary Medicine,2015,42(12):3119-3125. |
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| Authors: | QU Su-jie SHI Kai-chuang SU Yan-qiong HU Jie MO Sheng-lan ZHANG Bu-xian LI Jun ZOU Lian-bin |
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| Institution: | Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China |
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| Abstract: | To investigate genetic variation of avian infectious bronchitis virus (IBV) in Guangxi province,one strain of IBV was isolated from chicken.Two pairs of primers for amplifying the N and M genes of IBV were designed according to the sequences in GenBank.The N and M genes of the strain were amplified by RT-PCR,and they were proved to be the N and M genes of IBV by cloning,sequencing and compared with reference IBV strains published in GenBank.The results showed that the N gene from the IBV isolate consisted of 1 230 bp,coding 409 amino acids.The M gene from the IBV isolate consisted of 678 bp,coding 225 amino acids.The sequence analysis of N gene showed that it shared 87.2% to 93.3% nucleotide homologies and 90.0% to 94.4% deduced amino acid sequence homologies with IBV strains from GenBank.The M gene sequence analysis showed that it shared 83.6% to 91.0% nucleotide homologies and 82.7% to 92.9% deduced amino acid sequence homologies.The phylogenetic tree analysis showed that it was closely related to BJ and LX4 strains,and were clustered into one group;But with the distant relatives from other strains of IBV.These results suggested that the isolate was a new variant of IBV. |
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| Keywords: | avian infectious bronchitis virus N gene M gene gene clone sequence analysis |
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